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Product Info Summary
|Description:||Transient overexpression lysate of phosphorylase, glycogen; brain (PYGB)|
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GPBB (PYGB) (NM_002862) Human Over-expression Lysate
Transient overexpression lysate of phosphorylase, glycogen; brain (PYGB)
Storage & Handling
Cite This Product
GPBB (PYGB) (NM_002862) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005834)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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GPBB (PYGB) Human Over-expression Lysates NM_002862.
Western blot validation of overexpression lysate (LS005834) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PYGB (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for PYGB (Source: Uniprot.org, NCBI)
Glycogen phosphorylase, brain form
glycogen phosphorylase family
Brain glycogen phosphorylase; EC 126.96.36.199; Glycogen phosphorylase B; Glycogen phosphorylase brain form; Glycogen Phosphorylase Isoenzyme BB; GPBB; MGC9213; Phosphorylase glycogen brain; PYGB PYGB GPBB glycogen phosphorylase B glycogen phosphorylase, brain form|phosphorylase, glycogen; brain*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on PYGB, check out the PYGB Infographic
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In this infographic, you will see the following information for PYGB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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