HS3ST1 (NM_005114) Human Over-expression Lysate

Lysate of Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 over-expression cell lines, WB control

Transient overexpression lysate of heparan sulfate (glucosamine) 3-O-sulfotransferase 1 (HS3ST1)

Product Info Summary

SKU: LS009957
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of heparan sulfate (glucosamine) 3-O-sulfotransferase 1 (HS3ST1)

Customers Who Bought This Also Bought

Product Name

HS3ST1 (NM_005114) Human Over-expression Lysate

See all Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 products

SKU/Catalog Number

LS009957

Size

100 µg

Description

Transient overexpression lysate of heparan sulfate (glucosamine) 3-O-sulfotransferase 1 (HS3ST1)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

HS3ST1 (NM_005114) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009957)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for HS3ST1 (Source: Uniprot.org, NCBI)

Gene Name

HS3ST1

Full Name

Heparan sulfate glucosamine 3-O-sulfotransferase 1

Weight

35.773kDa

Superfamily

sulfotransferase 1 family

Alternative Names

3OST; 3OST1; 3-OST-1; 3OST1Heparan sulfate 3-O-sulfotransferase 1; EC 2.8.2; EC 2.8.2.23; h3-OST-1; heparan sulfate (glucosamine) 3-O-sulfotransferase 1; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1; heparan sulfate glucosamine 3-O-sulfotransferase 1; heparin-glucosamine 3-O-sulfotransferase; HS3ST1 HS3ST1 3OST, 3OST1 heparan sulfate-glucosamine 3-sulfotransferase 1 heparan sulfate glucosamine 3-O-sulfotransferase 1|3-OST-1|h3-OST-1|heparan sulfate (glucosamine) 3-O-sulfotransferase 1|heparan sulfate 3-O-sulfotransferase 1|heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1|heparin-glucosamine 3-O-sulfotransferase

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on HS3ST1, check out the HS3ST1 Infographic

HS3ST1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HS3ST1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for LS009957

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used HS3ST1 (NM_005114) Human Over-expression Lysate?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For HS3ST1 (NM_005114) Human Over-expression Lysate

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for HS3ST1 (NM_005114) Human Over-expression Lysate

$628
Backordered.

Lead time for this item is typically 2 weeks

Get A Quote
In stock
Order Product
LS009957
$628.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.