Human Aggrecan/ACAN PicoKine® Quick ELISA Kit

ACAN ELISA kit, 120-minute one-step protocol

Human Aggrecan PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ACAN in cell culture supernatants, serum and urine. Sensitivity: 50pg/ml.

Product Info Summary

SKU: FEK0909
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and urine.
Assay Time: 120 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human Aggrecan/ACAN PicoKine® Quick ELISA Kit

See all ACAN products

SKU/Catalog Number

FEK0909

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Aggrecan PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ACAN in cell culture supernatants, serum and urine. Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Aggrecan/ACAN PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0909)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: V20-R675

Sensitivity

<50 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0909 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0909 is reactive to ACAN in Human samples

Validated Sample Types

cell culture supernatants, serum and urine.

Application Guarantee

FEK0909 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of ACAN

Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.

Kit Components

Catalog number Description Quantity
FEK0909-CAP 96-well plate precoated with anti-Human ACAN antibody 1
EK0909-ST lyophilized recombinant Human ACAN standard 100 ng/tube
FEK0909-DA Biotinylated anti-Human ACAN detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)01.563.126.2512.52550100
O.D.0.0010.0520.1090.2350.5121.0331.7872.427

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3084130644310233221398240208
Standard deviation240.55679.321939.59275.72908.831809.36
CV (%)7.8%5.2%4.5%8.3%6.5%4.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ACAN in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13084301131912954306088.562.8%
Sample 21306411683113381303612280779.416.3%
Sample 34310243347442894284743396544.91.2%
*number of samples for each test n=16.

Gene/Protein Information For ACAN (Source: Uniprot.Org, NCBI)

Uniprot ID

P16112

Gene ID

176

Gene Name

ACAN

Full Name

Aggrecan core protein

Weight

261.329kDa

Superfamily

aggrecan/versican proteoglycan family

Alternative Names

AGC1SEDK; aggrecan core protein; aggrecan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; CSPG1aggrecan 1; CSPGCP; large aggregating proteoglycan; MSK16AGCAN ACAN AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK, SSOAOD aggrecan aggrecan core protein|cartilage-specific proteoglycan core protein|chondroitin sulfate proteoglycan core protein 1|large aggregating proteoglycan

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACAN, check out the ACAN Infographic

ACAN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACAN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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1 Customer Q&As for Human Aggrecan/ACAN PicoKine® Quick ELISA Kit

Question

I want to ask if this FEK0909 antibody stain in human cartilage on whether extracellular, pericellular region or intracellular region?

Verified Customer

Verified customer

Asked: 2014-07-18

Answer

The product FEK0909 have not been tested in staining of human cartilage. However, please check this publication for more information. PubMed: 12009333; Little CB et al. Matrix metalloproteinases are involved in C-terminal and inter-globular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002)

Boster Scientific Support

Answered: 2014-07-18

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