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Product Info Summary
| SKU: | PROTP15121 |
|---|---|
| Size: | Starting from 20ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
Human Aldose reductase Recombinant Protein
View all AKR1B1 recombinant proteins
SKU/Catalog Number
PROTP15121
Size
Starting from 20ug
Tag
Non-Tagged
Description
Human Aldose reductase Recombinant Protein expressed in E. coli (non-tagged). Sequence domain: 1-316aa. Application(s): SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
Human Aldose reductase Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP15121)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
35.9 kDa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Amino Acid Sequence
Human, P15121, 1-316aa; MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEFBioactivity
Specific activity is >800 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37°C.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
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