Human Annexin A1 PicoKine® Quick ELISA Kit

Annexin A1 ELISA kit, 90-minute one-step protocol

Human Annexin A1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANXA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 100pg/ml.

Product Info Summary

SKU: FEK1745
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Assay Time: 90 Minutes

How Quick Picokine(R) ELISA kits work: Get data in 90 minutes

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Product Name

Human Annexin A1 PicoKine® Quick ELISA Kit

See all Annexin A1 products

SKU/Catalog Number

FEK1745

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Annexin A1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANXA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 100pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Annexin A1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1745)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A2-N346

Sensitivity

<100 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1745 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1745 is reactive to ANXA1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Application Guarantee

FEK1745 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Annexin A1

ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca (2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca (2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

Kit Components

Catalog numberDescription QuantityVolume
FEK1745-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
EK1745-STHuman Annexin A1 Standard 2 10 ng/tube
FEK1745-AHuman ANXA1 Antibody A 1 3ml
FEK1745-BHuman ANXA1 Antibody B 1 3ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0560.1280.173 0.2740.4580.8331.4362.197

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2881452526330115635364
Standard deviation12.6759.53268.4117.16100.03413.03
CV (%)4.4%4.1%5.1%5.7%6.4%7.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Annexin A1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12882912942882906.392.2%
Sample 21452142613671348139827.972%
Sample 352634796510249825036125.892.5%
*number of samples for each test n=16.

Gene/Protein Information For ANXA1 (Source: Uniprot.Org, NCBI)

Gene Name

ANXA1

Full Name

Annexin A1

Weight

38.714kDa

Superfamily

annexin family

Alternative Names

Annexin A1; annexin I (lipocortin I); Annexin I; Annexin-1; ANX1; ANXA1; Calpactin II; calpactin-2; chromobindin-9; Lipocortin I; LPC1; LPC1annexin-1; p35; Phospholipase A2 inhibitory protein ANXA1 ANX1, LPC1 annexin A1 annexin A1|annexin I (lipocortin I)|annexin-1|calpactin II|calpactin-2|chromobindin-9|epididymis secretory sperm binding protein|phospholipase A2 inhibitory protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANXA1, check out the ANXA1 Infographic

ANXA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANXA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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