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Product Info Summary
| SKU: | EK1880 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human B4GALT1 ELISA Kit PicoKine®
View all beta-1,4-Galactosyltransferase 1/B4GalT1 ELISA kits
SKU/Catalog Number
EK1880
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human B4GALT1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human B4GALT1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human B4GALT1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1880)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G44-S398
Sensitivity
<50 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1880 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1880 is reactive to B4GALT1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1880 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of beta-1,4-Galactosyltransferase 1/B4GalT1
Beta-1,4-galactosyltransferase 1 is an enzyme that in humans is encoded by the B4GALT1 gene. It is mapped to 9p21.1. This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1880-CAP | Anti-Human B4GALT1 Pre-coated 96-well strip microplate | 1 |
| EK1880-ST | Human B4GALT1 Standard | 2 vials, 50 ng/tube |
| EK1880-DA | Human B4GALT1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.003 | 0.027 | 0.062 | 0.125 | 0.279 | 0.553 | 1.201 | 2.148 |
Data Example Images
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Human B4GALT1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect beta-1,4-Galactosyltransferase 1/B4GalT1, Dilution ratio of 1:5, concentration in serum and plasma is 3-30 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human B4GALT1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 258 | 1557 | 3706 | 299 | 1601 | 4232 |
| Standard deviation | 15.22 | 98.09 | 163.06 | 15.25 | 112.07 | 275.08 |
| CV (%) | 5.9% | 6.3% | 4.4% | 5.1% | 7.0% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of beta-1,4-Galactosyltransferase 1/B4GalT1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 258 | 272 | 274 | 299 | 275 | 14.77 | 5.3% |
| Sample 2 | 1557 | 1459 | 1502 | 1525 | 1510 | 35.69 | 2.3% |
| Sample 3 | 3706 | 4226 | 4110 | 3894 | 3984 | 199.88 | 5.0% |
Protein Target Info & Infographic
Gene/Protein Information For B4GALT1 (Source: Uniprot.Org, NCBI)
Gene Name
B4GALT1
Full Name
Beta-1,4-galactosyltransferase 1
Weight
43.92kDa
Superfamily
glycosyltransferase 7 family
Alternative Names
1,4- galactosyltransferase, polypeptide 1; B4GalT1; B4GAL-T1; beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; beta4Gal-T1; betaGlcNAc beta; CDG2D; EC 2.4.1; GGTB2; GGTB2DKFZp686N19253; glycoprotein-4-beta-galactosyltransferase 2; GT1; GTB; lactose synthase; MGC50983; UDP-Gal; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1 B4GALT1 B4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1 beta-1,4-galactosyltransferase 1 beta-1,4-galactosyltransferase 1|N-acetyllactosamine synthase|UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1|UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1|UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1|beta-1,4-GalTase 1|beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase|beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase|glycoprotein-4-beta-galactosyltransferase 2|lactose synthase A protein|nal synthase|neolactotriaosylceramide beta-1,4-galactosyltransferase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on B4GALT1, check out the B4GALT1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for B4GALT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human B4GALT1 ELISA Kit PicoKine® (EK1880)
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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10 Customer Q&As for Human B4GALT1 ELISA Kit PicoKine®
Question
Q: Can B4GalT1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
D. Nelson
Verified customer
Asked: 2020-11-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-11-16
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of B4GalT1 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-11-02
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the B4GalT1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-11-02
Question
Q: which procedure should I follow in order to thaw whole blood sample for B4GalT1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-03-28
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test B4GalT1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-03-28
Question
Q: how do I analyze ELISA data? I have performed B4GalT1 level in serum.
Verified Customer
Verified customer
Asked: 2018-11-24
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-11-24
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
W. Wu
Verified customer
Asked: 2018-10-20
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-20
Question
Q: What is the optimal O.D. value for B4GalT1 ELISA kit? I performed your B4GalT1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain B4GalT1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-04-28
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this B4GalT1 ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of B4GalT1 that can be declared/interpreted as positive by the above standard is 0.1ng/ml.
Boster Scientific Support
Answered: 2018-04-28
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification B4GalT1 using the EK1880 Human B4GalT1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-01-25
Question
Q: if the enzyme conjugated B4GalT1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the B4GalT1 antigen?
B. Mitchell
Verified customer
Asked: 2018-01-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-01-22
Question
Q: is it okay to use citrate plasma as samples in Human B4GalT1 Picokine® ELISA Kit (Catalog # EK1880)?
K. Tiwari
Verified customer
Asked: 2017-10-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of B4GalT1 causing degradation of its protein structure. B4GalT1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the B4GalT1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-10-17
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-01-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-01-21
