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Product Info Summary
| SKU: | EK1938 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. |
Product info
Product Name
Human CA3 ELISA Kit PicoKine®
View all Carbonic Anhydrase III/CA3 ELISA kits
SKU/Catalog Number
EK1938
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CA3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CA3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CA3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1938)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A2-K260
Sensitivity
<50 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1938 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1938 is reactive to CA3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.
Application Guarantee
EK1938 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Carbonic Anhydrase III/CA3
Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family that encode carbonic anhydrase isozymes. It is mapped to 8q21.2. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1938-CAP | Anti-Human CA3 Pre-coated 96-well strip microplate | 1 |
| EK1938-ST | Human CA3 Standard | 2 vials, 50 ng/tube |
| EK1938-DA | Human CA3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.035 | 0.066 | 0.111 | 0.199 | 0.380 | 0.671 | 1.105 | 1.803 |
Data Example Images
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Human CA3 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Carbonic Anhydrase III/CA3, Dilution ratio of 1:40, concentration in serum and plasma is around 300 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CA3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1301 | 6028 | 24574 | 1319 | 6108 | 24732 |
| Standard deviation | 53.34 | 373.74 | 1105.83 | 61.99 | 390.91 | 1335.53 |
| CV (%) | 4.1% | 6.2% | 4.5% | 4.7% | 6.4% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Carbonic Anhydrase III/CA3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1301 | 1297 | 1136 | 1156 | 1222 | 76.83 | 6.2% |
| Sample 2 | 6028 | 5779 | 6552 | 5475 | 5958 | 394.67 | 6.6% |
| Sample 3 | 24574 | 24410 | 24517 | 21425 | 23731 | 1332.95 | 5.6% |
Protein Target Info & Infographic
Gene/Protein Information For CA3 (Source: Uniprot.Org, NCBI)
Gene Name
CA3
Full Name
Carbonic anhydrase 3
Weight
29.557kDa
Superfamily
alpha-carbonic anhydrase family
Alternative Names
CA3; CAIII; CA-III; Car3; Carbonate dehydratase III; carbonic anhydrase 3; Carbonic Anhydrase III; carbonic anhydrase III, muscle specific; EC 4.2.1.1 CA3 CAIII, Car3 carbonic anhydrase 3 carbonic anhydrase 3|CA-III|HEL-S-167mP|carbonate dehydratase III|carbonic anhydrase III, muscle specific|carbonic anhydrase IIII|epididymis secretory sperm binding protein Li 167mP
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CA3, check out the CA3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CA3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CA3 ELISA Kit PicoKine® (EK1938)
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No publications found for EK1938
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Human CA3 ELISA Kit PicoKine®
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
C. Green
Verified customer
Asked: 2020-07-24
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-07-24
Question
Q: can you suggest the dilution ratio of serum samples for detection of CA3 in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-07-04
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CA3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-07-04
Question
Q: how do I analyze ELISA data? I have performed CA3 level in urine.
Verified Customer
Verified customer
Asked: 2019-11-03
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-03
Question
I want to use urine samples with EK1938, do you have suggestions on when it is the best time to collect the urine samples?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
For EK1938, Our lab technicians suggest to run a pilot test with urine samples collected in the afternoon.
Boster Scientific Support
Answered: 2019-10-10
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-04-19
Question
Q: how to thaw whole blood sample for CA3 ELISA after freezing?
B. Zhao
Verified customer
Asked: 2019-03-28
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CA3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-03-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Wilson
Verified customer
Asked: 2018-12-06
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-06
Question
Q: if the enzyme conjugated CA3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CA3 antigen?
Verified Customer
Verified customer
Asked: 2014-05-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-05-29
