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Product Info Summary
| SKU: | EK1306 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Cathepsin E ELISA Kit PicoKine®
View all Cathepsin E ELISA kits
SKU/Catalog Number
EK1306
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Cathepsin E ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTSE in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Cathepsin E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1306)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q18-P396
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1306 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1306 is reactive to CTSE in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1306 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cathepsin E
Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1306-CAP | Anti-Human CTSE Pre-coated 96-well strip microplate | 1 |
| EK1306-ST | Human CTSE Standard | 2 vials, 10 ng/tube |
| EK1306-DA | Human CTSE Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.019 | 0.103 | 0.165 | 0.280 | 0.501 | 0.908 | 1.472 | 2.188 |
Data Example Images
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Human Cathepsin E PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cathepsin E, Dilution ratio of 1:1, concentration in serum and plasma is around 500 pg/ml..
Some articles we found to cite concentrations of Cathepsin E in samples: 25348778 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Cathepsin E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 27 | 63 | 416 | 30 | 82 | 435 |
| Standard deviation | 1.30 | 3.59 | 19.14 | 1.53 | 4.84 | 27.84 |
| CV (%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cathepsin E in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 27 | 24 | 25 | 26 | 25 | 1.11 | 4.4% |
| Sample 2 | 63 | 61 | 69 | 71 | 66 | 4.12 | 6.2% |
| Sample 3 | 416 | 414 | 399 | 412 | 410 | 6.64 | 1.6% |
Protein Target Info & Infographic
Gene/Protein Information For CTSE (Source: Uniprot.Org, NCBI)
Gene Name
CTSE
Full Name
Cathepsin E
Weight
42.794kDa
Superfamily
peptidase A1 family
Alternative Names
CATE; Cathepsin E; CTSE; EC 3.4.23; EC 3.4.23.34; erythrocyte membrane aspartic proteinase; slow-moving proteinase CTSE CATE cathepsin E cathepsin E|erythrocyte membrane aspartic proteinase|slow-moving proteinase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CTSE, check out the CTSE Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CTSE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Cathepsin E ELISA Kit PicoKine® (EK1306)
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No publications found for EK1306
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Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human Cathepsin E ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human Cathepsin E Picokine® ELISA Kit (Catalog # EK1306)?
Verified Customer
Verified customer
Asked: 2020-10-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Cathepsin E causing disruption of its protein structure. Cathepsin E may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Cathepsin E ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-22
Question
Q: What is the optimal O.D. value for Cathepsin E ELISA kit? I used your Cathepsin E ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Cathepsin E even though the O.D. values are not very high?
V. Brown
Verified customer
Asked: 2020-07-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Cathepsin E ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Cathepsin E that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-07-05
Question
Q: how do I analyze ELISA data? I have obtained Cathepsin E level in plasma.
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-07-02
Question
Q: how can I thaw whole blood sample for Cathepsin E ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Cathepsin E for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-12-13
Question
Q: if the enzyme conjugated Cathepsin E antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cathepsin E antigen?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-11-15
Question
Q: Can Cathepsin E ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-09-28
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-09-28
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Cathepsin E using the EK1306 Human Cathepsin E Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2014-07-03
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2014-07-03
