Human CD163/M130 PicoKine® Quick ELISA Kit

CD163 ELISA kit, 90-minute one-step protocol

Human CD163 / M130 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 150pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: FEK0715
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin)
Assay Time: 90 Minutes

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Product Name

Human CD163/M130 PicoKine® Quick ELISA Kit

See all CD163 products

SKU/Catalog Number

FEK0715

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD163 / M130 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 150pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CD163/M130 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0715)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G41-S1045

Sensitivity

<150 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0715 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0715 is reactive to CD163 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin)

Application Guarantee

FEK0715 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CD163

CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.

Kit Components

Catalog numberDescription QuantityVolume
FEK0715-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
EK0715-STHuman CD163 Standard 2 100 ng/tube
FEK0715-AHuman CD163 Antibody A 1 3ml
FEK0715-BHuman CD163 Antibody B 1 3ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(ng/ml)01.563.126.2512.52550100
O.D.0.0110.2550.4050.7941.2911.8202.2052.478

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3520114564467433681129448653
Standard deviation144.32526.97303195.343195.34632.463357.05
CV (%)4.1%4.6%6.8%5.8%5.6%6.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD163 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135203403322230503298178.595.4%
Sample 21145611119132381207811972807.446.7%
Sample 344674429324230244756436661072.772.4%
*number of samples for each test n=16.

Gene/Protein Information For CD163 (Source: Uniprot.Org, NCBI)

Gene Name

CD163

Full Name

Scavenger receptor cysteine-rich type 1 protein M130

Weight

125.451kDa

Alternative Names

CD163 antigenscavenger receptor cysteine-rich type 1 protein M130; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; M130macrophage-associated antigen; MM130; RM3/1 CD163 M130, MM130, SCARI1 CD163 molecule scavenger receptor cysteine-rich type 1 protein M130|hemoglobin scavenger receptor|macrophage-associated antigen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD163, check out the CD163 Infographic

CD163 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD163: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

FEK0715 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

SIV Infection Impairs the Central Nervous System in Chinese Rhesus Macaques

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1 Customer Q&As for Human CD163/M130 PicoKine® Quick ELISA Kit

Question

Hello Support: I would like to know if this Human CD163 / M130 PicoKine

Verified Customer

Verified customer

Asked: 2018-03-08

Answer

The product Human CD163 / M130 PicoKine

Boster Scientific Support

Answered: 2018-03-08

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