Human CHRDL2 ELISA Kit PicoKine®

Chordin-like 2/CHRDL2 ELISA kit for Human

Human CHRDL2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CHRDL2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2168
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

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Product Name

Human CHRDL2 ELISA Kit PicoKine®

View all Chordin-like 2/CHRDL2 ELISA kits

SKU/Catalog Number

EK2168

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CHRDL2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CHRDL2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CHRDL2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2168)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-T429

Sensitivity

<10 pg/ml

Assay Range

93.7 pg/ml - 6,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2168 is reactive to CHRDL2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2168 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Chordin-like 2/CHRDL2

This gene encodes a member of the chordin family of proteins. Chordin family members are secreted proteins that share a cysteine-rich pro-collagen repeat domain and associate with members of the transforming growth factor beta superfamily. In vitro assays demonstrate a direct interaction between the encoded protein and human activin A. This gene is expressed in many tissues including osteoblasts, where it is differentially expressed during differentiation. In addition, its expression is upregulated in human osteoarthritic joint cartilage, suggesting a role in adult cartilage regeneration.

Kit Components

Catalog Number Description Quantity
EK2168-CAP Anti-Human CHRDL2 Pre-coated 96-well strip microplate 1
EK2168-ST Human CHRDL2 Standard 2 vials, 10 ng/tube
EK2168-DA Human CHRDL2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)093.7187.53757501,5003,0006,000
O.D.0.0570.1200.2580.5310.7671.0821.6192.199

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CHRDL2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)102332844118432941
Standard deviation5.4114.9443.045.0722.0344.23
CV(%)5.3%4.5%5.1%4.3%5.1%4.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Chordin-like 2/CHRDL2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11021221311421245.474.4%
Sample 233236537729634317.475.1%
Sample 3844912101095493049.295.3%
*number of samples for each test n=16.

Gene/Protein Information For CHRDL2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q6WN34

Gene ID

25884

Gene Name

CHRDL2

Full Name

Chordin-like protein 2

Weight

47.495kDa

Alternative Names

BNF-1; Chordin like 2; Chordin-like 2; chordin-like protein 2; CHRDL2 CHRDL2 BNF1, CHL2 chordin like 2 chordin-like protein 2|breast tumor novel factor 1|chordin-related protein 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CHRDL2, check out the CHRDL2 Infographic

CHRDL2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CHRDL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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