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Product Info Summary
| SKU: | PROTP04080 |
|---|---|
| Size: | Starting from 10ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
Human Cystatin B Recombinant Protein
View all CSTB recombinant proteins
SKU/Catalog Number
PROTP04080
Size
Starting from 10ug
Tag
His-Tag
Description
Human Cystatin B Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-98aa. Application(s): Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
Human Cystatin B Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP04080)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.0) containing 50mM NaCl
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
11.1 kDa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Amino Acid Sequence
Human, P04080, 1-98aa; MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYFBioactivity
The IC50 value is < 3.0nM. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Specific Publications For Human Cystatin B Recombinant Protein (PROTP04080)
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