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Product Info Summary
| SKU: | EK0749 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Decorin / DCN ELISA Kit PicoKine®
SKU/Catalog Number
EK0749
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Decorin / DCN ELISA Kit PicoKine™ (96 Tests). Quantitate Human DCN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Decorin / DCN ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0749)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G17-K359
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0749 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0749 is reactive to DCN in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0749 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DCN
Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size.It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0749-CAP | Anti-Human DCN Pre-coated 96-well strip microplate | 1 |
| EK0749-ST | Human DCN Standard | 2 vials, 10 ng/tube |
| EK0749-DA | Human DCN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.001 | 0.063 | 0.129 | 0.322 | 0.620 | 1.124 | 1.697 | 2.059 |
Data Example Images
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Human Decorin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect DCN, Dilution ratio of 1:1, concentration in serum and plasma is around 2 ng/ml..
Some articles we found to cite concentrations of DCN in samples: 23026977, 23607294 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Decorin / DCN ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 60 | 247 | 1001 | 56 | 270 | 1067 |
| Standard deviation | 4.56 | 16.54 | 70.07 | 4.31 | 20.25 | 88.56 |
| CV (%) | 7.6% | 6.7% | 7% | 7.7% | 7.5% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DCN in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 51 | 50 | 59 | 55 | 4.52 | 8.2% |
| Sample 2 | 247 | 279 | 302 | 287 | 278 | 20.1 | 7.2% |
| Sample 3 | 1001 | 962 | 922 | 976 | 965 | 28.61 | 2.9% |
Protein Target Info & Infographic
Gene/Protein Information For DCN (Source: Uniprot.Org, NCBI)
Gene Name
DCN
Full Name
Decorin
Weight
39.747kDa
Superfamily
small leucine-rich proteoglycan (SLRP) family
Alternative Names
Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B DCN CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B decorin decorin|bone proteoglycan II|dermatan sulphate proteoglycans II|proteoglycan core protein|small leucine-rich protein 1B
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DCN, check out the DCN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DCN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Decorin / DCN ELISA Kit PicoKine® (EK0749)
Hello CJ!
EK0749 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Elucidating Decorin’s role in the preovulatory follicle
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
20 Customer Q&As for Human Decorin / DCN ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-08-03
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-03
Question
Q: Can DCN ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
E. Tiwari
Verified customer
Asked: 2020-07-20
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-07-20
Question
Q: which procedure should I follow in order to thaw whole blood sample for DCN ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test DCN for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-01-20
Question
Has EK0749 been validated to detect the protein in its native form (if it has glycosaminoglycan chains on it)?
Verified customer
Asked: 2020-01-17
Answer
The Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) has been validated to detect the protein in its native form. However, our lab hasn't validated if it has glycosaminoglycan chains on it.
Boster Scientific Support
Answered: 2020-01-19
Question
Is EK0749 designed for XYZ species or is it designed for a different species such as human and cross reacts with XYZ species?
Verified customer
Asked: 2020-01-08
Answer
The reactivity for Human Decorin / DCN ELISA Kit PicoKine EK0749 is human and no detectable cross reactivity.
Boster Scientific Support
Answered: 2020-01-09
Question
Is the capture antibody for EK0749 monoclonal or polyclonal?
Verified customer
Asked: 2020-01-07
Answer
The capture antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Monoclonal.
Boster Scientific Support
Answered: 2020-01-09
Question
What is the host of the capture antibody for EK0749?
Verified customer
Asked: 2020-01-07
Answer
The host of the capture antibody of the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Mouse.
Boster Scientific Support
Answered: 2020-01-09
Question
What is the species of the immunogen capture antibody for EK0749, Human or something else?
Verified customer
Asked: 2020-01-07
Answer
The species for the immunogen capture antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Human.
Boster Scientific Support
Answered: 2020-01-09
Question
What is the host of the detection antibody for EK0749?
Verified customer
Asked: 2020-01-07
Answer
The host of the detection antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Mouse.
Boster Scientific Support
Answered: 2020-01-09
Question
What is the species of the immunogen detection antibody for EK0749? Human or something else?
Verified customer
Asked: 2020-01-07
Answer
The species of the immunogen detection antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Human.
Boster Scientific Support
Answered: 2020-01-09
Question
What is the standard for EK0749? A recombinant protein or something else? If recombinant protein, what is the host?
Verified customer
Asked: 2020-01-07
Answer
For the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749), the standard is a recombinant protein and the host is Human.
Boster Scientific Support
Answered: 2020-01-09
Question
Is the recombinant protein standard for EK0749 full length or partial?
Verified customer
Asked: 2020-01-07
Answer
The recombinant protein standard for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Full G17-K359
Boster Scientific Support
Answered: 2020-01-09
Question
What is the species of the standard for EK0749? Human or something else?
Verified customer
Asked: 2020-01-07
Answer
The species of the standard for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Human.
Boster Scientific Support
Answered: 2020-01-09
Question
What sample type was tested with EK0749 (Serum, Plasma, or something else?) and what were the reference values?
Verified customer
Asked: 2020-01-07
Answer
The tested sample types for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) are Cell Culture Supernates, Cell Lysates, Serum And Plasma (Heparin, EDTA). Anticoagulant(s): heparin or EDTA.
Boster Scientific Support
Answered: 2020-01-09
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Jackson
Verified customer
Asked: 2019-07-20
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-07-20
Question
One of our customers is interested in purchasing the kit EK0749, qty 5. Would you mind confirming the lead time and pricing? Is 1 lot of qty 5 available?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
We do not have 5 kits of the same lot available. It takes around 3 business days for manufacturing it after we receive the order.
Boster Scientific Support
Answered: 2019-06-21
Question
What is the immunogen or immunogen range (partial or full-length) of the capture antibody for EK0749?
Verified customer
Asked: 2019-01-07
Answer
The immunogen or immunogen range of the capture antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is G17-K359 (Accession num. P07585).
Boster Scientific Support
Answered: 2019-01-09
Question
Q: if the enzyme conjugated DCN antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the DCN antigen?
Y. Evans
Verified customer
Asked: 2018-09-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-09-05
Question
Q: is it okay to use citrate plasma as samples in Human DCN Picokine® ELISA Kit (Catalog # EK0749)?
S. Huang
Verified customer
Asked: 2017-04-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of DCN causing degradation of its protein structure. DCN may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the DCN ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-04-29
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
K. Huang
Verified customer
Asked: 2016-01-18
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-01-18
