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Product Info Summary
| SKU: | PROTP00374 |
|---|---|
| Size: | Starting from 20ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
Human Dihydrofolate Reductase/DHFR Recombinant Protein
View all DHFR recombinant proteins
SKU/Catalog Number
PROTP00374
Size
Starting from 20ug
Tag
His-Tag
Description
Human Dihydrofolate Reductase/DHFR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-187aa. Application(s): SDS-PAGE, Enzyme Activity.
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
Human Dihydrofolate Reductase/DHFR Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP00374)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
21.5 kDa
Amino Acid Sequence
Human, P00374, 1-187aa;Bioactivity
Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Specific Publications For Human Dihydrofolate Reductase/DHFR Recombinant Protein (PROTP00374)
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