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Product Info Summary
| SKU: | FEK0867 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human DKK-1/Dickkopf 1 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0867
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human DKK-1/Dickkopf 1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human DKK-1/Dickkopf 1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human DKK-1/Dickkopf 1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0867)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: T32-H266
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0867 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0867 is reactive to DKK1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK0867 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Dkk-1
Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0867-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0867-ST | Human Dkk-1 Standard | 2 | 10 ng/tube |
| FEK0867-A | Human DKK1 Antibody A | 1 | 3ml |
| FEK0867-B | Human DKK1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.033 | 0.062 | 0.171 | 0.253 | 0.425 | 0.733 | 1.291 | 1.902 |
Data Example Images
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Human DKK1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human DKK-1/Dickkopf 1 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 166 | 652 | 1518 | 147 | 564 | 1364 |
| Standard deviation | 9.79 | 29.34 | 103.224 | 8.67 | 25.38 | 92.75 |
| CV(%) | 5.9% | 4.5% | 6.8% | 5.8% | 4.5% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Dkk-1 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 166 | 155 | 167 | 152 | 160 | 3.52 | 2.2% |
| Sample 2 | 652 | 694 | 637 | 671 | 664 | 31.85 | 4.8% |
| Sample 3 | 1518 | 1503 | 1246 | 1377 | 1411 | 63.5 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For DKK1 (Source: Uniprot.Org, NCBI)
Gene Name
DKK1
Full Name
Dickkopf-related protein 1
Weight
29.298kDa
Superfamily
dickkopf family
Alternative Names
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like Dkk1|mdkk-1|dickkopf WNT signaling pathway inhibitor 1|dickkopf-related protein 1|dickkopf homolog 1|dickkopf-1|dickkopf-like protein 1|dkk-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DKK1, check out the DKK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DKK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human DKK-1/Dickkopf 1 PicoKine® Quick ELISA Kit (FEK0867)
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