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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
|Assay Time:||90 Minutes|
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Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Human FASL / CD95LG / CD95 Ligand PicoKine™ Quick ELISA Kit
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human FASL / CD95LG / CD95 Ligand PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human FASL / CD95LG / CD95 Ligand PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0337)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: CHO; Immunogen sequence: P134-L281
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
FEK0337 is reactive to FASLG in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
FEK0337 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|FEK0337-CAP||96-well plate precoated with anti-Human Fas Ligand/TNFSF6 antibody||1|
|FEK0337-ST||lyophilized recombinant Human Fas Ligand/TNFSF6 standard||10 ng/tube|
|FEK0337-DA||Biotinylated anti-Human Fas Ligand/TNFSF6 detection antibody (50x)||130 ul|
|-||Avidin-Biotin-Peroxidase Complex (30x)||400 µl|
|AR1106-1||Sample diluent||30 ml|
|AR1106-2||Antibody diluent||12 ml|
|-||Avidin-Biotin-Peroxidase Diluent||12 ml|
|AR1106-5||Wash Buffer (25x)||20 ml|
|AR1104||Color Developing Reagent (TMB)||10 ml|
|AR1105||Stop Solution||10 ml|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human FASL PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Fas Ligand/TNFSF6 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FASLG (Source: Uniprot.Org, NCBI)
Tumor necrosis factor ligand superfamily member 6
tumor necrosis factor family
apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6 FASLG ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, TNFSF6, TNLG1A Fas ligand tumor necrosis factor ligand superfamily member 6|CD95 ligand|Fas ligand (TNF superfamily, member 6)|apoptosis (APO-1) antigen ligand 1|apoptosis antigen ligand|fas antigen ligand|mutant tumor necrosis factor family member 6|tumor necrosis factor ligand 1A*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FASLG, check out the FASLG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FASLG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for FEK0337
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