Human FASL / CD95LG / CD95 Ligand PicoKine™ Quick ELISA Kit

Human FASL / CD95LG / CD95 Ligand PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml.

Product Info Summary

SKU: FEK0337
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Assay Time: 90 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human FASL / CD95LG / CD95 Ligand PicoKine™ Quick ELISA Kit

See all Fas Ligand/TNFSF6 products

SKU/Catalog Number

FEK0337

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human FASL / CD95LG / CD95 Ligand PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human FASL / CD95LG / CD95 Ligand PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0337)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: P134-L281

Sensitivity

<2 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0337 is reactive to FASLG in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

FEK0337 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity
FEK0337-CAP 96-well plate precoated with anti-Human Fas Ligand/TNFSF6 antibody 1
FEK0337-ST lyophilized recombinant Human Fas Ligand/TNFSF6 standard 10 ng/tube
FEK0337-DA Biotinylated anti-Human Fas Ligand/TNFSF6 detection antibody (50x) 130 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0080.1140.1960.3790.7401.3712.1212.693

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3618643734189419
Standard deviation2.199.6728.842.112.4733.1
CV (%)6.1%5.2%6.6%6.2%6.6%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Fas Ligand/TNFSF6 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 136414134383.088.1%
Sample 21861971921981934.762.4%
Sample 343749544650747130.216.4%
*number of samples for each test n=16.

Gene/Protein Information For FASLG (Source: Uniprot.Org, NCBI)

Uniprot ID

P48023

Gene ID

356

Gene Name

FASLG

Full Name

Tumor necrosis factor ligand superfamily member 6

Weight

31.485kDa

Superfamily

tumor necrosis factor family

Alternative Names

apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6 FASLG ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, TNFSF6, TNLG1A Fas ligand tumor necrosis factor ligand superfamily member 6|CD95 ligand|Fas ligand (TNF superfamily, member 6)|apoptosis (APO-1) antigen ligand 1|apoptosis antigen ligand|fas antigen ligand|mutant tumor necrosis factor family member 6|tumor necrosis factor ligand 1A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FASLG, check out the FASLG Infographic

FASLG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FASLG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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1 Customer Q&As for Human FASL / CD95LG / CD95 Ligand PicoKine™ Quick ELISA Kit

Question

An inquiry: For this antibody FEK0337 at where the FasL protein do the antibodies bind? I want to measure the amount of a chimeric FasL molecule I have made versus the WT FasL protein. The chimeric protein contains AA 139-281 of human trans-membrane FasL. It also has an HA tag so I could just use an antibody against the HA tag if necessary. Do you have any other ELISA kit that I will use against HA tag?

Verified Customer

Verified customer

Asked: 2013-08-12

Answer

The epitopes of this FEK0337 antibody is not known, however, the antibody pair is binding somewhere on the extracellular domain (aa 134-281). The capture antibody is monoclonal and the detection antibody is polyclonal. As for HA tag we do not have a kit against HA though we have many antibodies that are anti-HA.

Boster Scientific Support

Answered: 2013-08-12

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