Human GAPDH Recombinant Protein

GAPDH protein,

Human GAPDH Recombinant Protein expressed in E. coli (non-tagged). Sequence domain: 1-335aa. Application(s): SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

Product Info Summary

SKU: PROTP04406
Size: Starting from 4ug
Source: E. coli
Application: Enzyme Activity, SDS-PAGE

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Product Name

Human GAPDH Recombinant Protein

View all GAPDH recombinant proteins

SKU/Catalog Number

PROTP04406

Size

Starting from 4ug

Tag

Non-Tagged

Description

Human GAPDH Recombinant Protein expressed in E. coli (non-tagged). Sequence domain: 1-335aa. Application(s): SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

Storage & Handling

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Human GAPDH Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP04406)

Form

Liquid

Formulation

20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 1mM EDTA, 20% glycerol

Concentration

1mg/ml (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Predicted MW

36.053kDa

Amino Acid Sequence

Human, P04406, 1-335aa; MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE

Bioactivity

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37°C.

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Validation Images & Assay Conditions

Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)

Gene Name

GAPDH

Full Name

Glyceraldehyde-3-phosphate dehydrogenase

Weight

36.053kDa

Superfamily

glyceraldehyde-3-phosphate dehydrogenase family

Alternative Names

1D4 GAPDH; aging-associated gene 9 protein; Cytoplasm Marker; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PD; G3PDH; GAPD; GAPDH; GAPDPeptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38; OCT1 coactivator in S phase; Peptidyl-cysteine S-nitrosylase GAPDH GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on GAPDH, check out the GAPDH Infographic

GAPDH infographic

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In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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SKU:PROTP04406

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