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Product Info Summary
| SKU: | PROTP04406 |
|---|---|
| Size: | Starting from 4ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
Human GAPDH Recombinant Protein
View all GAPDH recombinant proteins
SKU/Catalog Number
PROTP04406
Size
Starting from 4ug
Tag
Non-Tagged
Description
Human GAPDH Recombinant Protein expressed in E. coli (non-tagged). Sequence domain: 1-335aa. Application(s): SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
Human GAPDH Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP04406)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 1mM EDTA, 20% glycerol
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
36.1 kDa
Amino Acid Sequence
Human, P04406, 1-335aa; MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKEBioactivity
Specific activity is > 50 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37°C.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Specific Publications For Human GAPDH Recombinant Protein (PROTP04406)
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