Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human GDF15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ0767
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all GDF-15 products

SKU/Catalog Number

EZ0767

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human GDF15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0767)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: A197-I308

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0767 is reactive to GDF15 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EZ0767 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0767-CA Rabbit anti- human GDF15 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0767-DA Biotinylated goat anti- human GDF15 polyclonal antibody (Detection Antibody) 500 µl, 1 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0767-ST Lyophilized recombinant human GDF15 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0020.1060.2260.4490.7941.3571.9972.479

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)532521068532661115
Standard deviation3.5516.1274.763.9220.2181.39
CV (%)6.7%6.4%7%7.4%7.6%7.3%

Gene/Protein Information For GDF15 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q99988

Gene ID

9518

Gene Name

GDF15

Full Name

Growth/differentiation factor 15

Weight

34.14kDa

Superfamily

TGF-beta family

Alternative Names

GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta GDF15 GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB growth differentiation factor 15 growth/differentiation factor 15|NRG-1|NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1|NSAID-activated gene 1 protein|NSAID-regulated gene 1 protein|PTGF-beta|macrophage inhibitory cytokine 1|non-steroidal anti-inflammatory drug-activated gene-1|placental TGF-beta|placental bone morphogenetic protein|prostate differentiation factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GDF15, check out the GDF15 Infographic

GDF15 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GDF15: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

What is the recommended sample diluent for EZ0767?

Verified customer

Asked: 2019-05-09

Answer

The recommended sample diluent of the Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0767) for serum is 1:1.

Boster Scientific Support

Answered: 2019-05-10

Question

Do you recommend to include 0.05% Tween 20 v/v in the wash buffer and do you foresee any issue?

Verified customer

Asked: 2019-05-09

Answer

For the Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0767), you can include 0.05% Tween 20 v/v in the wash buffer. It is OK to use 1x PBS with NaCl, KCl, Na2HPO4 and KH2PO4.

Boster Scientific Support

Answered: 2019-05-10

Question

Is washing recommended after coating (before blocking) for EZ0767?

Verified customer

Asked: 2019-05-09

Answer

For the Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0767), washing or not washing would not make too much difference to the assay result.

Boster Scientific Support

Answered: 2019-05-10

Question

Do you recommend washing step after sample/standard/control incubation int the assay procedure for EZ0767?

Verified customer

Asked: 2019-05-09

Answer

No, we do not recommend washing step after sample/standard/control incubation for the Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0767).

Boster Scientific Support

Answered: 2019-05-10

Question

Would you recommend using PBS pH7.2 – 7.4 or Sodium Bicarb buffer pH 9.6 for EZ0767?

Verified customer

Asked: 2019-05-09

Answer

Both PBS pH7.2 – 7.4 and Sodium Bicarb buffer pH 9.6 are commonly used capture antibody diluent. Our lab hasn't validated their performance. If you prefer, please run a pilot test to validate their effect. We recommend using "Capture Antibody Diluent: 100 mL H2O, 0.5 g Na2HPO4", which is stated on our datasheet.

Boster Scientific Support

Answered: 2019-05-10

Question

Is there pH requirement for “Capture Antibody Diluent: 100 mL H2O, 0.5 g Na2HPO4”?

Verified customer

Asked: 2019-05-07

Answer

The pH value should be 8.0-9.0.

Boster Scientific Support

Answered: 2019-05-07

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