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Product Info Summary
| SKU: | EK0362 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine®
SKU/Catalog Number
EK0362
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine™ (96 Tests). Quantitate Human GDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0362)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: R109-I211
Sensitivity
<4 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0362 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0362 is reactive to GDNF in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0362 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GDNF
Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0362-CAP | Anti-Human GDNF Pre-coated 96-well strip microplate | 1 |
| EK0362-ST | Human GDNF Standard | 2 vials, 10 ng/tube |
| EK0362-DA | Human GDNF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.020 | 0.127 | 0.226 | 0.425 | 0.943 | 1.273 | 1.502 | 2.061 |
Data Example Images
Click image to see more details
Human GDNF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 69 | 264 | 855 | 66 | 242 | 810 |
| Standard deviation | 4.96 | 17.16 | 36.76 | 6 | 16.45 | 37.26 |
| CV (%) | 7.2% | 6.5% | 4.3% | 9.1% | 6.8% | 4.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GDNF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 69 | 76 | 77 | 70 | 73 | 3.53 | 4.8% |
| Sample 2 | 264 | 272 | 267 | 262 | 266 | 3.76 | 1.4% |
| Sample 3 | 855 | 943 | 952 | 812 | 890 | 59.07 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For GDNF (Source: Uniprot.Org, NCBI)
Gene Name
GDNF
Full Name
Glial cell line-derived neurotrophic factor
Weight
23.72kDa
Superfamily
TGF-beta family
Alternative Names
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3 GDNF ATF, ATF1, ATF2, HFB1-GDNF, HSCR3 glial cell derived neurotrophic factor glial cell line-derived neurotrophic factor|astrocyte-derived trophic factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GDNF, check out the GDNF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GDNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine® (EK0362)
Hello CJ!
EK0362 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells
Serum glial cell line-derived neurotrophic factor levels and impulsivity in heroin addiction: a cross-sectional, case-control study of 129 heroin addicts
Exercise improves neurotrophins in multiple sclerosis independent of disability status
A synergistic effect between family intervention and rTMS improves cognitive and negative symptoms in schizophrenia: A randomized controlled trial
Serum brain-derived neurotrophic factor, glial-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 levels in children with attention-deficit/hyperactivity disorder
H19 regulates the proliferation of bovine male germline stem cells via IGF‐1 signaling pathway
Ahmed MN,Shi D,Dailey MT,Rothermund K,Drewry MD,Calabrese TC,Cui XT,Syed-Picard FN. Dental pulp cell sheets enhance facial nerve regeneration via local neurotrophic factor delivery. Tissue Eng Part A.2020 Nov 9. doi:10.1089/ten.TEA.2020.0265.Epub ahead of
Species: Human
Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells
Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.
Yang C, Zhou L, Gao X, Chen B, Tu J, Sun H, Liu X, He J, Liu J, Yuan Q. Neurosurgery. 2011 Mar;68(3):691-704. Doi: 10.1227/Neu.0B013E3182098A8A. Neuroprotective Effects Of Bone Marrow Stem Cells Overexpressing Glial Cell Line-Derived Neurotrophic ...
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
10 Customer Q&As for Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Human GDNF Picokine® ELISA Kit (Catalog # EK0362)?
Verified Customer
Verified customer
Asked: 2020-08-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of GDNF causing degradation of its protein structure. GDNF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the GDNF ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-08-09
Question
Q: What is the optimal O.D. value for GDNF ELISA kit? I used your GDNF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain GDNF even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-05-31
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GDNF ELISA kit to have sensitivity of 4pg/ml, that means the minimum amount of GDNF that can be declared/interpreted as positive by the above standard is 4pg/ml.
Boster Scientific Support
Answered: 2020-05-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-11-28
Question
Q: how to analyze ELISA data? I measured GDNF level in cell lysates.
S. Jha
Verified customer
Asked: 2019-04-10
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-04-10
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification GDNF using the EK0362 Human GDNF Picokine® ELISA Kit?
D. Dhar
Verified customer
Asked: 2018-04-29
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-04-29
Question
Q: if the enzyme conjugated GDNF antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GDNF antigen?
W. Jackson
Verified customer
Asked: 2018-02-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-02-22
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
A. Dayal
Verified customer
Asked: 2017-10-23
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-10-23
Question
Q: how to thaw whole blood sample for GDNF ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-04-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test GDNF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-04-11
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of GDNF in Human cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2016-02-01
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the GDNF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-02-01
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
C. Perez
Verified customer
Asked: 2014-05-10
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-05-10
