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Product Info Summary
| SKU: | PROTQ16775 |
|---|---|
| Size: | Starting from 10ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
Human Glyoxalase II Recombinant Protein
View all HAGH recombinant proteins
SKU/Catalog Number
PROTQ16775
Size
Starting from 10ug
Tag
His-Tag
Description
Human Glyoxalase II Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-260aa. Application(s): Enzyme Activity, SDS-PAGE.
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
Human Glyoxalase II Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ16775)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.5) containing 10% glycerol
Concentration
0.5mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
33.8 kDa
Amino Acid Sequence
Human, Q16775, 1-260aa; MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRDBioactivity
Specific activity is > 500 units/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37°C.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
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