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Product Info Summary
| SKU: | EK0376 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IGF-1 ELISA Kit PicoKine®
View all IGF-I/IGF-1 ELISA kits
SKU/Catalog Number
EK0376
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IGF-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IGF1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IGF-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0376)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: G49-A118
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0376 for more details
Cross-reactivity
There is no detectable cross-reactivity with IGF-2.
Reactive Species
EK0376 is reactive to IGF1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0376 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGF-I/IGF-1
Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. It is mapped to 12q23.2. The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0376-CAP | Anti-Human IGF1 Pre-coated 96-well strip microplate | 1 |
| EK0376-ST | Human IGF1 Standard | 2 vials, 10 ng/tube |
| EK0376-DA | Human IGF1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.043 | 0.081 | 0.137 | 0.215 | 0.406 | 0.714 | 1.226 | 1.817 |
Data Example Images
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Human IGF-1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGF-I/IGF-1, Dilution ratio of 1:4, concentration in serum and plasma is around 2-6 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IGF-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 215 | 1065 | 3566 | 284 | 1154 | 4861 |
| Standard deviation | 8.82 | 45.80 | 164.04 | 14.48 | 68.09 | 325.69 |
| CV (%) | 4.1% | 4.3% | 4.6% | 5.1% | 5.9% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IGF-I/IGF-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 215 | 211 | 191 | 188 | 201 | 11.88 | 5.9% |
| Sample 2 | 1065 | 1091 | 1134 | 1073 | 1090 | 26.68 | 2.4% |
| Sample 3 | 3566 | 3691 | 3369 | 3307 | 3483 | 153.39 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For IGF1 (Source: Uniprot.Org, NCBI)
Gene Name
IGF1
Full Name
Insulin-like growth factor I
Weight
21.841kDa
Superfamily
insulin family
Alternative Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C IGF1 IGF, IGF-I, IGFI, MGF insulin like growth factor 1 insulin-like growth factor I|insulin-like growth factor 1 (somatomedin C)|insulin-like growth factor IB|mechano growth factor|somatomedin-C
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IGF1, check out the IGF1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IGF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IGF-1 ELISA Kit PicoKine® (EK0376)
Hello CJ!
EK0376 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin
Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer
Resistance to multikinase inhibitor actions mediated by insulin like growth factor-1
Elevated expression levels of serum insulin‐like growth factor‐1, tumor necrosis factor‐α and vascular endothelial growth factor 165 might exacerbate type 2 diabetic nephropathy
Expression and localization of insulin-like growth factor-I in four parts of the red deer antler
Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial
The dynamic changes of circulating OCN+ cells versus insulinlike growth factor-I during primary healing of orthognathic surgeries
Elementary research into the transformation BmN cells mediated by the piggyBac transposon vector
Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic hIGF-I silkworms
Adipose tissue extract shows potential for wound healing: in vitro proliferation and migration of cell types contributing to wound healing in the presence of adipose tissue preparation and platelet rich plasma
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
10 Customer Q&As for Human IGF-1 ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
J. Bhasin
Verified customer
Asked: 2020-11-13
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-11-13
Question
Would it would work with their bear samples?
Verified customer
Asked: 2020-09-25
Answer
The animal species trying to measure IGF1 concentrations is the Asiatic black bear (Ursus thibetanus Japonicus), and the exact protein sequencing has not been reported. In the case of closely related species, brown and polar bears have genomic information available to the public.
Boster Scientific Support
Answered: 2020-10-25
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
S. Anand
Verified customer
Asked: 2020-07-23
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-07-23
Question
I am doing a research about human IGF-1. I would like to know whether EK0376 Human IGF-1 ELISA Kit can detect free of total IGF-1. I really need your help. Thank you
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The EK0376 Human IGF-1 ELISA Kit is designed to measure free IGF1. IGF-1 is usually bound to IGFBPs in the serum/plasma, so if you would like to measure total IGF1, it is advisable to release the analytes by performing an extraction procedure such as acid-ethanol treatment.
Boster Scientific Support
Answered: 2020-05-08
Question
I am interested purchasing EK0376 Human IGF-1 ELISA Kit for serum or plasma samples. I read the protocol calls for pre-treatment of samples to separate IGF1 from the IGF1 Binding Proteins in order to accurately measure total IGF1. Is it possible not performing the extraction step and only measure free IGF1 (not initially bound to IGF1BPs) in serum or plasma? ? or have you ever tried it?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
Hello; the purpose of the extraction step is to increase the signal, as the antibody EK0376 Human IGF-1 ELISA Kit recognition is apparently blocked by the binding protein interfering with the epitopes that one or both of the antibodies bind to. Probably some signal will be obtained even without the extraction step, but the EK0376 Human IGF-1 ELISA Kit will not be able to differentiate signal contributed by free EGF1 from the signal contributed by EGF1 in complex with binding protein.
Boster Scientific Support
Answered: 2019-12-23
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IGF-I using the EK0376 Human IGF-I Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-10-08
Question
Q: can you recommend the dilution ratio of serum samples for detection of IGF-I in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
J. Babu
Verified customer
Asked: 2018-03-31
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IGF-I ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-03-31
Question
Q: how to proceed with the analysis of ELISA data? I have performed IGF-I level in plasma.
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-08-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2015-12-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-12-02
Question
Q: if the enzyme conjugated IGF-I antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IGF-I antigen?
Verified Customer
Verified customer
Asked: 2015-03-28
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2015-03-28
