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Product Info Summary
| SKU: | EK0579 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
Product info
Product Name
Human LIF ELISA Kit PicoKine®
SKU/Catalog Number
EK0579
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LIF PicoKine ELISA Kit® (96 Tests). Quantitate Human LIF in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 8 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LIF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0579)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S23-F202
Sensitivity
<8 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0579 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0579 is reactive to LIF in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Application Guarantee
EK0579 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LIF
Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0579-CAP | Anti-Human LIF Pre-coated 96-well strip microplate | 1 |
| EK0579-ST | Human LIF Standard | 2 vials, 10 ng/tube |
| EK0579-DA | Human LIF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.015 | 0.038 | 0.155 | 0.261 | 0.424 | 0.816 | 1.234 | 2.028 |
Data Example Images
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Human LIF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LIF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 23 | 40 | 341 | 30 | 54 | 431 |
| Standard deviation | 0.6 | 2.14 | 18.96 | 0.79 | 1.73 | 18.02 |
| CV(%) | 2.6% | 5.3% | 5.6% | 2.6% | 3.2% | 4.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of LIF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 23 | 27 | 16 | 16 | 21 | 1.12 | 5.5% |
| Sample 2 | 40 | 51 | 32 | 47 | 43 | 3.2 | 7.5% |
| Sample 3 | 341 | 484 | 449 | 426 | 425 | 27.71 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For LIF (Source: Uniprot.Org, NCBI)
Gene Name
LIF
Full Name
Leukemia inhibitory factor
Weight
22.008kDa
Superfamily
LIF/OSM family
Alternative Names
CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI LIF CDF, DIA, HILDA, MLPLI LIF interleukin 6 family cytokine leukemia inhibitory factor|D factor|cholinergic differentiation factor|differentiation inhibitory activity|differentiation-inducing factor|differentiation-stimulating factor|hepatocyte-stimulating factor III|human interleukin in DA cells|melanoma-derived LPL inhibitor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LIF, check out the LIF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LIF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human LIF ELISA Kit PicoKine® (EK0579)
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