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- Table of Contents
Lcn2 antibody (PB9608) performs well in ELISA, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).
Lipocalin 2 was detected in paraffin-embedded section of Mouse Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Lipocalin 2 was detected in paraffin-embedded section of Mouse Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-Lipocalin 2/LCN2 Antibody Picoband®
Lcn2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
Lcn2 antibody was positively detected by WB in:
mouse spleen tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Lcn2 antibody was positively detected by IHC in:
Mouse Spleen tissue, Rat Spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Lcn2 antibody was positively detected by IHC-F in:
mouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
ELISA
0.1-0.5μg/ml
Western blot
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Mouse
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Lipocalin 2/LCN2 Antibody Picoband®
View all LCN2 Antibodies
View all LCN2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Lipocalin 2 recombinant protein (Position: Q21-N200). Mouse Lipocalin 2 shares 62 % and 81.1 % amino acid (aa) sequence identity with human and rat Lipocalin 2, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
22.9 kDa
Background of Lcn2
Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.
Cite This Product
Anti-Lipocalin 2/LCN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9608)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
81%
Moderate Homology
Rabbit
68%
Low homology
Monkey
62%
Low homology
Human
62%
Low homology
Sheep
61%
Low homology
Dog
61%
Low homology
Cow
59%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Lcn2 antibody
Immunohistochemistry (IHC) protocol for Lcn2 antibody
Publications
Application:
Species:
Gandouling Regulates Ferroptosis and Improves Neuroinflammation in Wilson’s Disease Through the LCN2/NLRP3 Signaling Pathway
Published: 2024-08-23
Journal: Journal of Inflammation Research
Application: WB
Impact Factor: 4.20
Species: Mouse
PMID: 39193124
Dexmedetomidine alleviates lipopolysaccharide-induced acute kidney injury by inhibiting the NLRP3 inflammasome activation via regulating the TLR4/NOX4/NF-κB pathway
Published: 2019-06-26
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 3.45
Species: Rat
PMID: 31243816
Peri-ovarian adipose tissue contributes to intraovarian control during folliculogenesis in mice
Published: 2018-08-01
Journal: REPRODUCTION
Application: WB
Impact Factor: 3.09
Species: Mouse
PMID: 29844226
Expression of neutrophil gelatinase‑associated lipocalin in low osmolar contrast‑induced nephropathy in rats and the effect of N‑acetylcysteine
Published: 2016-10-04
Journal: Experimental and Therapeutic Medicine
Application: IHC
Impact Factor: 1.28
Species: Rat
PMID: 27882134
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Product Promise
We promise Anti-Lipocalin 2/LCN2 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9608
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026