Human LOX-1/OLR1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human LOX-1/OLR1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human OLR1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Product Info Summary

SKU: EZ0824
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human LOX-1/OLR1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all LOX-1/OLR1 products

SKU/Catalog Number

EZ0824

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human LOX-1/OLR1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human OLR1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human LOX-1/OLR1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0824)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S61-Q273

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0824 is reactive to OLR1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Application Guarantee

EZ0824 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0824-CA Rabbit anti- human OLR1 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0824-DA Biotinylated goat anti- human OLR1 polyclonal antibody (Detection Antibody) 500 µl, 2.5 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0824-ST Lyophilized recombinant human OLR1 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0220.1010.1760.3410.6051.0681.5622.285

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)23681912373269281528
Standard deviation12.515059.421.1966.8284.04
CV (%)5.3%6.1%4.8%6.5%7.2%5.5%

Gene/Protein Information For OLR1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P78380

Gene ID

4973

Gene Name

OLR1

Full Name

Oxidized low-density lipoprotein receptor 1

Weight

30.959kDa

Alternative Names

CLEC8A; CLEC8ASLOX1; C-type lectin domain family 8 member A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX1; LOX-1; LOX1ox LDL receptor 1; LOXIN; OLR1; oxidised low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1, soluble form; Ox-LDL receptor 1; SCARE1; scavenger receptor class E, member 1; SR-E1 OLR1 CLEC8A, LOX1, LOXIN, SCARE1, SLOX1 oxidized low density lipoprotein receptor 1 oxidized low-density lipoprotein receptor 1|C-type lectin domain family 8 member A|hLOX-1|lectin-type oxidized LDL receptor 1|ox LDL receptor 1|oxidized low density lipoprotein (lectin-like) receptor 1|oxidized low-density lipoprotein receptor 1, soluble form|scavenger receptor class E, member 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on OLR1, check out the OLR1 Infographic

OLR1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for OLR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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