- Table of Content
Human PD-L1/B7-H1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD274 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 12pg/ml. Cited in 2 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Assay Range:||62.5 pg/ml - 4,000 pg/ml|
|Product Name||Human PD-L1/B7-H1 ELISA Kit PicoKine™
See more PD-L1 products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Human PD-L1/B7-H1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD274 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 12pg/ml.|
|Cite This Product||Human PD-L1/B7-H1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1449)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
|Assay Range||62.5 pg/ml - 4,000 pg/ml|
|Immunogen/Standard||Expression system for standard: NS0; Immunogen sequence: F19-R238|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||29126|
|Species Of This Entry||Human|
|Protein Name||Programmed cell death 1 ligand 1|
|Alternative Names||PD-L1|B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for CD274: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1449-CAP||Anti-Human CD274 Pre-coated 96-well strip microplate||1|
|EK1449-ST||Human CD274 Standard||2 vials, 10 ng/tube|
|EK1449-DA||Human CD274 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK1449 Human PD-L1/B7-H1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human PD-L1/B7-H1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of PD-L1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Here are featured tools and databases that you might find useful.
|PD-L1 Ameliorates Murine Acute Graft-Versus-Host Disease by Suppressing Effector But Not Regulatory T Cells Function||PubMed ID 30927016|
|Liu ZH, Zheng FF, Mao YL, Ye LF, Bian J, Lai DH, Ye YL, Dai YP. Oncol Lett. 2017 Jun;13(6):4818-4824. doi: 10.3892/ol.2017.6080. Epub 2017 Apr 24. Effects of programmed death-ligand 1 expression on OK-432 immunotherapy following transurethral rese...||PubMed ID 28599483|
Domestically in USA and Canada, ships next business day if available in stock.
Comprehensive info on CD274, beautifully presented.Open Infographic
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
We promise all of our products perform as described in datasheets.