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Product Info Summary
| SKU: | EK0469 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Human Beta Nerve Growth Factor / NGF Beta / NGFB ELISA Kit PicoKine®
SKU/Catalog Number
EK0469
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Beta Nerve Growth Factor / NGF Beta / NGFB ELISA Kit PicoKine™ (96 Tests). Quantitate Human NGF in cell culture supernatants and serum. Sensitivity: 15 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Beta Nerve Growth Factor / NGF Beta / NGFB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0469)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S122-A241
Sensitivity
<15 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0469 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0469 is reactive to NGF in Human samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0469 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of beta-NGF
Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0469-CAP | Anti-Human NGF Pre-coated 96-well strip microplate | 1 |
| EK0469-ST | Human NGF Standard | 2 vials, 10 ng/tube |
| EK0469-DA | Human NGF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.062 | 0.091 | 0.133 | 0.225 | 0.403 | 0.735 | 1.165 | 2.059 |
Data Example Images
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Human NGF/NGF beta PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect beta-NGF, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 31.2 pg/ml..
Some articles we found to cite concentrations of beta-NGF in samples: 24691584 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Beta Nerve Growth Factor / NGF Beta / NGFB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 50 | 99 | 673 | 57 | 106 | 839 |
| Standard deviation | 1.05 | 5.62 | 40.65 | 3.51 | 8.14 | 56.13 |
| CV(%) | 2.1% | 5.7% | 6% | 6.2% | 7.7% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of beta-NGF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 50 | 52 | 40 | 60 | 51 | 2.29 | 4.5% |
| Sample 2 | 99 | 89 | 107 | 76 | 93 | 3.79 | 4.1% |
| Sample 3 | 673 | 660 | 887 | 1000 | 805 | 33.97 | 4.2% |
Protein Target Info & Infographic
Gene/Protein Information For NGF (Source: Uniprot.Org, NCBI)
Gene Name
NGF
Full Name
Beta-nerve growth factor
Weight
26.959kDa
Superfamily
NGF-beta family
Alternative Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 NGF Beta-NGF, HSAN5B, NGF nerve growth factor beta-nerve growth factor|nerve growth factor (beta polypeptide)|nerve growth factor, beta subunit|pro-nerve growth factor long
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NGF, check out the NGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Beta Nerve Growth Factor / NGF Beta / NGFB ELISA Kit PicoKine® (EK0469)
Hello CJ!
EK0469 has been cited in 24 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Improvement of postoperative cognitive dysfunction and attention network function of patients with ischemic cerebrovascular disease via dexmedetomidine
Nerve Growth Factor Serum Levels Are Associated With Regional Gray Matter Volume Differences in Schizophrenia Patients
Associations between proinflammatory cytokines in the synovial fluid and radiographic grading and pain-related scores in 47 consecutive patients with osteoarthritis of the knee
Effect of Sertraline, Dosulepin, and Venlafaxine on Non-BDNF Neurotrophins in Patients With Depression: A Cohort Study
Nerve growth factor regulates the proliferation of cashmere goat outer root sheath cells through the activation of cAMP-binding protein
Oriented co-continuous 3D porous scaffolds with inhibited activating functionality: An effective strategy to inhibit the hyperactivation of astrocytes
Effect of lurasidone vs olanzapine on neurotrophic biomarkers in unmedicated schizophrenia: A randomized controlled trial
Gestational acrylamide exposure and biomarkers of fetal growth: Probing the mechanism underlying the association between acrylamide and reduced fetal growth
Effects of cryopreservation with a newly-developed magnetic field programmed freezer on periodontal ligament cells and pulp tissues
Human umbilical cord Whartons jelly-derived mesenchymal stem cells differentiate into a Schwann-cell phenotype and promote neurite outgrowth in vitro
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
8 Customer Q&As for Human Beta Nerve Growth Factor / NGF Beta / NGFB ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-03-16
Question
Does this human ELISA kit have any cross reactivity with rat and mouse samples?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
This kit has about 2.5% cross reactivity with rat sample. For mouse sample, the cross reactivity is about 30%.
Boster Scientific Support
Answered: 2020-02-27
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-04-23
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-04-23
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-03-31
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-03-31
Question
Q: if the enzyme conjugated beta-NGF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the beta-NGF antigen?
K. Nelson
Verified customer
Asked: 2018-02-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-02-09
Question
Q: What is the optimal O.D. value for beta-NGF ELISA kit? I used your beta-NGF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain beta-NGF even though the O.D. values are not very high?
P. Dayal
Verified customer
Asked: 2017-05-11
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this beta-NGF ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of beta-NGF that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2017-05-11
Question
Q: how to analyze ELISA data? I have performed beta-NGF level in serum.
Verified Customer
Verified customer
Asked: 2017-03-24
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-03-24
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of beta-NGF in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Y. Chakrabarti
Verified customer
Asked: 2017-02-02
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the beta-NGF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-02-02
