Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA Kit

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA Kit

See all Tnfrsf11b products

SKU/Catalog Number

FEK0481

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Tnfrsf11b in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

TNFRSF11B (osteoprotegerin/OPG) is a soluble TNF-receptor–family decoy that inhibits osteoclastogenesis and helps regulate bone resorption; it is also described as an independent risk factor for atherosclerosis progression/cardiovascular disease. Assay context: 120-minute ‘Quick’ sandwich ELISA for mouse Tnfrsf11b in cell-culture supernatants, serum, and EDTA plasma; sensitivity <5 pg/mL; assay range 93.7–6,000 pg/mL; capture mAb (rat) + detection pAb (goat) with stated lack of detectable cross-reactivity. Often interpreted alongside matrix/ECM remodeling readouts such as COL1A1 (Collagen I) and stromal remodeling factors like POSTN (Periostin) (putative), and integrated with broader cytokine panels in multiplex assays or transcript-level confirmation via qPCR.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0481)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: E22-L401

Sensitivity

<5 pg/ml

Assay Range

93.7 pg/ml - 6,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0481 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0481 is reactive to Tnfrsf11b in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA).

Application Guarantee

FEK0481 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Tnfrsf11b

Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system.

Kit Components

Catalog number	Description	Quantity
FEK0481-CAP	96-well plate precoated with anti-Mouse Tnfrsf11b antibody	1
EK0481-ST	lyophilized recombinant Mouse Tnfrsf11b standard	10 ng/tube
FEK0481-DA	Biotinylated anti-Mouse Tnfrsf11b detection antibody (50x)	100 ul
-	Avidin-Biotin-Peroxidase Complex (30x)	400 µl
AR1106-1	Sample diluent	30 ml
AR1106-2	Antibody diluent	12 ml
-	Avidin-Biotin-Peroxidase Diluent	12 ml
AR1106-5	Wash Buffer (25x)	20 ml
AR1104	Color Developing Reagent (TMB)	10 ml
AR1105	Stop Solution	10 ml
PLA-SEA	Plate Sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	93.7	187.5	375	750	13000	3000	6000
O.D.	0.000	0.082	0.173	0.294	0.522	0.976	1.585	2.118

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Gene/Protein Information For Tnfrsf11b (Source: Uniprot.Org, NCBI)

Alternative Names

Tumor necrosis factor receptor superfamily member 11B;Osteoclastogenesis inhibitory factor;Osteoprotegerin;Tnfrsf11b;Ocif, Opg;

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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