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Product Info Summary
| SKU: | EK0897 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. |
Product info
Product Name
Human PLAT/TPA ELISA Kit PicoKine®
View all t-Plasminogen Activator/tPA ELISA kits
SKU/Catalog Number
EK0897
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PLAT/TPA ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLAT in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PLAT/TPA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0897)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: M1-P562
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0897 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0897 is reactive to PLAT in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
Application Guarantee
EK0897 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of t-Plasminogen Activator/tPA
Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0897-CAP | Anti-Human PLAT Pre-coated 96-well strip microplate | 1 |
| EK0897-ST | Human PLAT Standard | 2 vials, 10 ng/tube |
| EK0897-DA | Human PLAT Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
| O.D. | 0.013 | 0.093 | 0.177 | 0.29 | 0.764 | 1.091 | 1.82 | 2.441 |
Data Example Images
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Human PLAT/TPA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect t-Plasminogen Activator/tPA, Dilution ratio of 1:20, concentration in serum and plasma is around 30 ng/ml..
Some articles we found to cite concentrations of t-Plasminogen Activator/tPA in samples: 17532723 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PLAT/TPA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 84 | 237 | 975 | 81 | 229 | 878 |
| Standard deviation | 5.37 | 15.4 | 43.87 | 5.58 | 17.63 | 43.02 |
| CV (%) | 6.4% | 6.5% | 4.5% | 6.9% | 7.7% | 4.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of t-Plasminogen Activator/tPA in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 84 | 72 | 75 | 77 | 77 | 4.41 | 5.7% |
| Sample 2 | 237 | 271 | 264 | 232 | 251 | 16.77 | 6.6% |
| Sample 3 | 975 | 906 | 909 | 870 | 915 | 37.88 | 4.1% |
Protein Target Info & Infographic
Gene/Protein Information For PLAT (Source: Uniprot.Org, NCBI)
Gene Name
PLAT
Full Name
Tissue-type plasminogen activator
Weight
62.917kDa
Superfamily
peptidase S1 family
Alternative Names
Alteplase; DKFZp686I03148; EC 3.4.21; EC 3.4.21.68; plasminogen activator, tissue type; plasminogen activator, tissue; PLAT; Reteplase; tissue plasminogen activator (t-PA); tissue-type plasminogen activator; TPA; T-PA; tPlasminogen Activator; t-Plasminogen Activator PLAT T-PA, TPA plasminogen activator, tissue type tissue-type plasminogen activator|alteplase|plasminogen/activator kringle|reteplase|t-plasminogen activator
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PLAT, check out the PLAT Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLAT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PLAT/TPA ELISA Kit PicoKine® (EK0897)
Hello CJ!
EK0897 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Improved Antithrombotic Function of Oriented Endothelial Cell Monolayer on Microgrooves
Temperature-Gating Titania Nanotubes Regulate Migration of Endothelial Cells
Effective-constituent compatibility-based analysis of Bufei Yishen formula, a traditional herbal compound as an effective treatment for chronic obstructive pulmonary disease
Oligodeoxyribonucleotides derived from salmon sperm DNA: An alternative to defibrotide
Mechanical Adaptability of the MMP‐Responsive Film Improves the Functionality of Endothelial Cell Monolayer
Mechanical Adaptability of the MMP‐Responsive Film Improves the Functionality of Endothelial Cell Monolayer
Mechanical Adaptability of the MMP‐Responsive Film Improves the Functionality of Endothelial Cell Monolayer
Hu, M., Chang, H., Zhang, H., Wang, J., Lei, W.X., Li, B.C.,…, & Ji, J. (2017). Mechanical Adaptability of the MMP-Responsive Film Improves the Functionality of Endothelial Cell Monolayer. Advanced Healthcare Materials. Advance online publication....
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
8 Customer Q&As for Human PLAT/TPA ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Adams
Verified customer
Asked: 2020-08-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-08
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-10-14
Question
Q: can you recommend the dilution ratio of serum samples for detection of tPA in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the tPA ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-09-30
Question
Q: which procedure should I follow in order to thaw whole blood sample for tPA ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test tPA for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-06-21
Question
Does the kit also determine mouse tPA? Can it detect Alteplase in mouse blood? Keyword: applications, clinical
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
No, it can't recognize mouse TPA. The standard in EK0897 is recombinant human PLAT(CHO, M1-P562). The kit can recognize the Alteplase in theory if the sequence is included. But it also depends on the constitution of the recombinant protein. Pilot assay is recommended first.
Boster Scientific Support
Answered: 2019-02-08
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Phillips
Verified customer
Asked: 2018-10-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-10-25
Question
Q: What is the optimal O.D. value for tPA ELISA kit? I performed your tPA ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain tPA even though the O.D. values are not very high?
W. Martinez
Verified customer
Asked: 2016-05-17
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this tPA ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of tPA that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2016-05-17
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement tPA using the EK0897 Human tPA Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2014-06-05
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2014-06-05
