Human Resistin PicoKine® Quick ELISA Kit

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human Resistin PicoKine® Quick ELISA Kit

See all RETN products

SKU/Catalog Number

FEK0581

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Resistin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human RETN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

RETN (resistin) is described as a hormone-like factor that can suppress insulin-stimulated glucose uptake in adipose cells (by similarity) and is often discussed in obesity–metabolic inflammation contexts (canonical functional framing). Assay context: 120-minute “Quick” sandwich ELISA for Human RETN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) with sensitivity 3 pg/mL; metabolic-inflammation panels often interpret RETN alongside inflammatory cytokine axes such as IL1B (putative) and vascular/RAAS physiology markers such as ACE (putative cross-pathway context).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Resistin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0581)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: K19-P108

Capture/Detection Antibodies

The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.

Sensitivity

<3 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0581 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0581 is reactive to RETN in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0581 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of RETN

Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

Kit Components

Catalog number	Description	Quantity
FEK0581-CAP	96-well plate precoated with anti-Human RETN antibody	1
EK0581-ST	lyophilized recombinant Human RETN standard	10 ng/tube
FEK0581-DA	Biotinylated anti-Human RETN detection antibody (50x)	100 ul
-	Avidin-Biotin-Peroxidase Complex (30x)	400 µl
AR1106-1	Sample diluent	30 ml
AR1106-2	Antibody diluent	12 ml
-	Avidin-Biotin-Peroxidase Diluent	12 ml
AR1106-5	Wash Buffer (25x)	20 ml
AR1104	Color Developing Reagent (TMB)	10 ml
AR1105	Stop Solution	10 ml
PLA-SEA	Plate Sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.013	0.169	0.306	0.483	0.926	1.391	1.920	2.305

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Resistin PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Gene/Protein Information For RETN (Source: Uniprot.Org, NCBI)

Alternative Names

Resistin;Adipose tissue-specific secretory factor;ADSF;C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein;Cysteine-rich secreted protein A12-alpha-like 2;Cysteine-rich secreted protein FIZZ3;RETN;FIZZ3, HXCP1, RSTN;UNQ407/PRO1199;

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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