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Product Info Summary
| SKU: | EK1501 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine®
View all Sphingosine Kinase 1/SPHK1 ELISA kits
SKU/Catalog Number
EK1501
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SPHK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1501)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: M1-L384
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1501 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1501 is reactive to SPHK1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1501 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Sphingosine Kinase 1/SPHK1
Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1- phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1501-CAP | Anti-Human SPHK1 Pre-coated 96-well strip microplate | 1 |
| EK1501-ST | Human SPHK1 Standard | 2 vials, 20 ng/tube |
| EK1501-DA | Human SPHK1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 200000000 | 20000000 | 2000000 | 200000 | 20000 |
| O.D. | 0.013 | 0.062 | 0.125 | 0.223 | 0.465 | 0.841 | 1.547 | 2.216 |
Data Example Images
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Human SPHK1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Sphingosine Kinase 1/SPHK1, Dilution ratio of 1:1, concentration in serum and plasma is 0-720 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 530 | 1744 | 8630 | 561 | 1890 | 8353 |
| Standard deviation | 25.44 | 104.64 | 576.1%%1 | 35.34 | 114.8%9 | 651.53 |
| CV (%) | 4.8% | 6% | 6.7% | 6.1%% | 6.1% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Sphingosine Kinase 1/SPHK1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 530 | 551 | 501 | 466 | 512 | 31.94 | 6.2% |
| Sample 2 | 1744 | 1935 | 1605 | 1844 | 1782 | 122.5 | 6.8% |
| Sample 3 | 8630 | 9352 | 9453 | 8421 | 8964 | 446.11 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For SPHK1 (Source: Uniprot.Org, NCBI)
Gene Name
SPHK1
Full Name
Sphingosine kinase 1
Weight
42.518kDa
Alternative Names
EC 2.7.1.91; SK1; sphingosine kinase 1; SPHK1; SPHKSK 1; SPK 1; SPK; SPK1 SPHK1 SPHK sphingosine kinase 1 sphingosine kinase 1|SK 1|SPK 1|acetyltransferase SPHK1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SPHK1, check out the SPHK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SPHK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine® (EK1501)
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No publications found for EK1501
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine®
Question
Q: can you recommend the dilution ratio of serum samples for detection of SPHK1 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-07-31
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SPHK1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-07-31
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring SPHK1 using the EK1501 Human SPHK1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-02-14
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-02-14
Question
Q: Can SPHK1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
K. Jones
Verified customer
Asked: 2016-09-21
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-09-21
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-09-15
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-09-15
Question
Q: What is the optimal O.D. value for SPHK1 ELISA kit? I used your SPHK1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SPHK1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-03-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SPHK1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of SPHK1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2016-03-05
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
A. Zhou
Verified customer
Asked: 2015-12-13
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-12-13
