Human sVEGFR1/sFLT1 ELISA Kit PicoKine™

Human sVEGFR1/sFLT1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FLT1 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 30pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0543
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (EDTA).

Product Name

Human sVEGFR1/sFLT1 ELISA Kit PicoKine™

See all VEGFR1/Flt-1 products

SKU/Catalog Number

EK0543

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human sVEGFR1/sFLT1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FLT1 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 30pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human sVEGFR1/sFLT1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0543)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S27-H687

Sensitivity

<30 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0543 is reactive to FLT1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA).

Application Guarantee

EK0543 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0543-CAP Anti-Human FLT1 Pre-coated 96-well strip microplate 1
EK0543-ST Human FLT1 Standard 2 vials, 10 ng/tube
EK0543-DA Human FLT1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0310.0690.1240.1640.3660.6811.1781.920

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect VEGFR1/Flt-1, Dilution ratio of 1:1, concentration in serum is 3.6-5ng/ml target in serum is below detection limit..

Some articles we found to cite concentrations of VEGFR1/Flt-1 in samples: 16449946 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human sVEGFR1/sFLT1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2371369442024013524316
Standard deviation9.71106.78203.3211.28131.14202.85
CV (%)4.1%7.8%4.6%4.7%9.7%4.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of VEGFR1/Flt-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12372122282282269.013.9%
Sample 21369131813261352134120.361.5%
Sample 344204343459847794535168.513.7%
*number of samples for each test n=16.

Gene/Protein Information For FLT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P17948

Gene ID

2321

Gene Name

FLT1

Full Name

Vascular endothelial growth factor receptor 1

Weight

150.769kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1 FLT1 FLT, FLT-1, VEGFR-1, VEGFR1 fms related receptor tyrosine kinase 1 vascular endothelial growth factor receptor 1|fms related tyrosine kinase 1|fms-like tyrosine kinase 1|fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)|tyrosine-protein kinase FRT|tyrosine-protein kinase receptor FLT|vascular permeability factor receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FLT1, check out the FLT1 Infographic

FLT1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FLT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0543 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu M,Wang RB,Xing JH,Tang YX.Nested Case–Control Study of Corin Combined with sFlt-1/PLGF in Predicting the Risk of Preeclampsia.Int J Gen Med.2021;14:2313-2320https://doi.org/10.2147/IJGM.S297344
Species: Human
EK0543 usage in article: APP:ELISA, SAMPLE: SERUM, DILUTION:NA

Eroglu E, Kocyigit I, Kahriman G, Karakukcu C, Tuncay A, Zararsiz GE, Eren D, Kalay N, Sipahioglu MH, Oymak O, Tokgoz B. Soluble vascular endothelial growth factor receptor-1 as a novel marker of arteriovenous fistula stenosis in hemodialysis patients. He
Species: Human
EK0543 usage in article: APP:ELISA, SAMPLE:HUMAN SERUM, DILUTION:1:10

Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene

Liu J, Li J, Su C, Huang B, Luo S. Acta Biochim Biophys Sin (Shanghai). 2007 Jul;39(7):499-506. Soluble Fms-Like Tyrosine Kinase-1 Expression Inhibits The Growth Of Multiple Myeloma In Nude Mice.

Bakir M, Bakir S, Sari I, Celik Vk, Gozel Mg, Engin A. J Med Virol. 2013 Oct;85(10):1794-801. Doi: 10.1002/Jmv.23666. Epub 2013 Jul 19. Evaluation Of The Relationship Between Serum Levels Of Vegf And Svegfr1 With Mortality And Prognosis In Patient...

Have you used Human sVEGFR1/sFLT1 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human sVEGFR1/sFLT1 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

13 Customer Q&As for Human sVEGFR1/sFLT1 ELISA Kit PicoKine™

Question

What serum dilution do you suggest for measuring sFlt1 in serum samples of severe preclamptic and eclamptic patients using EK0543?

Verified customer

Asked: 2020-11-12

Answer

For the Human sVEGFR1/sFLT1 ELISA Kit (EK0543), our lab tested serum from normal healthy humans with dilution of 1:1. We suggest starting with 1:1. Please, run pilot tests with his specific samples to figure out the optimal dilution ratio.

Boster Scientific Support

Answered: 2020-11-13

Question

Does EK0543 react with rat and mouse samples?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

EK0543 will not react with rat and mouse samples.

Boster Scientific Support

Answered: 2020-03-04

Question

Q: Can VEGFR1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

T. Liu

Verified customer

Asked: 2019-08-03

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-08-03

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

B. Wright

Verified customer

Asked: 2019-04-09

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-04-09

Question

Will EK0543 also react with Rat sFLT1?

Verified customer

Asked: 2019-03-12

Answer

The Human SVEGFR1/SFLT1 ELISA Kit PicoKine™ (EK0543) will not react with rat sFLT1.

Boster Scientific Support

Answered: 2019-03-12

Question

Q: can you recommend the dilution ratio of serum samples for detection of VEGFR1 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-01-11

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the VEGFR1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-01-11

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

S. Miller

Verified customer

Asked: 2018-09-09

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-09-09

Question

Do you know if EK0543 will react with rat and mouse samples?

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

EK0543 will not react with rat and mouse samples.

Boster Scientific Support

Answered: 2018-04-19

Question

a customer who purchased EK0543 requests if you have any suggestion or how many hours after the blood collection they should proceed with serum separation in order to analyze the samples using your kit?

Verified Customer

Verified customer

Asked: 2017-10-12

Answer

It takes four hours

Boster Scientific Support

Answered: 2017-10-12

Question

Why is there such a large concentration range of 156-10000 pg/ml for the Human sVEGFR1/sFLT1 PicoKine™ ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-10-12

Answer

The range has been set to take into account that this detects a growth factor

Boster Scientific Support

Answered: 2017-10-12

Question

Does EK0543 detected bound or unbound VEGF-A?

Verified Customer

Verified customer

Asked: 2017-07-27

Answer

EK0543 is able to detect both bound and unbound SVEGFR1/SFLT1.

Boster Scientific Support

Answered: 2017-07-27

Question

Q: is it okay to use heparin plasma as samples in Human VEGFR1 Picokine® ELISA Kit (Catalog # EK0543)?

Verified Customer

Verified customer

Asked: 2016-09-24

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of VEGFR1 causing degradation of its protein structure. VEGFR1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the VEGFR1 ELISA, treating samples with various anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-09-24

Question

Q: which procedure should I follow in order to thaw whole blood sample for VEGFR1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-09-02

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VEGFR1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-09-02

$370

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK0543
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.