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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (EDTA).|
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Human VEGFR1/sFLT1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human VEGFR1/sFLT1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FLT1 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 30pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human VEGFR1/sFLT1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0543)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: S27-H687
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0543 is reactive to FLT1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
EK0543 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFR1/Flt-1
sVEGFR1, also known as sFMS-related tyrosine kinase 1 (sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.
|EK0543-CAP||Anti-Human FLT1 Pre-coated 96-well strip microplate||1|
|EK0543-ST||Human FLT1 Standard||2 vials, 10 ng/tube|
|EK0543-DA||Human FLT1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human VEGFR1/sFLT1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VEGFR1/Flt-1, Dilution ratio of 1:1, concentration in serum is 3.6-5ng/ml target in serum is below detection limit..
Some articles we found to cite concentrations of VEGFR1/Flt-1 in samples: 16449946 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human VEGFR1/sFLT1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of VEGFR1/Flt-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FLT1 (Source: Uniprot.Org, NCBI)
Vascular endothelial growth factor receptor 1
protein kinase superfamily
EC 2.7.10; EC 126.96.36.199; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1 FLT1 FLT, FLT-1, VEGFR-1, VEGFR1 fms related receptor tyrosine kinase 1 vascular endothelial growth factor receptor 1|fms related tyrosine kinase 1|fms-like tyrosine kinase 1|fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)|tyrosine-protein kinase FRT|tyrosine-protein kinase receptor FLT|vascular permeability factor receptor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FLT1, check out the FLT1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FLT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0543 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum Vascular Endothelial Growth Factor and Vascular Endothelial Growth Factor Receptor-1 Levels in Patients With Fibromyalgia Syndrome
Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene
Attenuation of hyperglycemia-induced apoptotic signaling and anti-angiogenic milieu in cultured cytotrophoblast cells
Soluble Fms‐like Tyrosine Kinase‐1 Expression Inhibits the Growth of Multiple Myeloma in Nude Mice
Soluble vascular endothelial growth factor receptor‐1 as a novel marker of arteriovenous fistula stenosis in hemodialysis patients
Vascular endothelial growth factor gene polymorphisms in patients with rosacea: A case-control study
Protective effect of human umbilical cord mesenchymal stem cell exosomes on preserving the morphology and angiogenesis of placenta in rats with preeclampsia
Upregulation of sFlt-1 by trophoblasts induces the barrier dysfunction of glomerular endothelial cells
Effects of maternal serum on permeability of glomerular endothelial cell membrane
Liu M,Wang RB,Xing JH,Tang YX.Nested Case–Control Study of Corin Combined with sFlt-1/PLGF in Predicting the Risk of Preeclampsia.Int J Gen Med.2021;14:2313-2320https://doi.org/10.2147/IJGM.S297344
EK0543 usage in article: APP:ELISA, SAMPLE: SERUM, DILUTION:NA
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