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Product Info Summary
| SKU: | FEK0523 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin , EDTA) and saliva |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human TIMP3 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0523
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TIMP3 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human TIMP3 in cell culture supernatants, serum, plasma (heparin , EDTA) and saliva. Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TIMP3 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0523)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: C24-P211
Sensitivity
<2 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0523 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0523 is reactive to TIMP3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin , EDTA) and saliva
Application Guarantee
FEK0523 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TIMP-3
The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0523-CAP | Anti-Human TIMP-3 Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0523-ST | Human TIMP-3 Standard | 2 | 10 ng/tube |
| FEK0523-H | HRP Linked Anti-Human TIMP3 Antibody | 1 | 6ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.111 | 0.160 | 0.190 | 0.231 | 0.414 | 1.003 | 2.177 | 2.388 |
Data Example Images
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Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 228 | 1364 | 4394 | 244 | 1487 | 4066 |
| Standard deviation | 14.59 | 90.02 | 237.27 | 19.03 | 107.06 | 333.41 |
| CV (%) | 6.4% | 6.6% | 5.4% | 7.8% | 7.2% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TIMP-3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 228 | 257 | 260 | 256 | 250 | 11.25 | 4.5% |
| Sample 2 | 1364 | 1309 | 1336 | 1522 | 1382 | 81.53 | 5.9% |
| Sample 3 | 4394 | 4601 | 4288 | 4589 | 4468 | 285.95 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For TIMP3 (Source: Uniprot.Org, NCBI)
Gene Name
TIMP3
Full Name
Metalloproteinase inhibitor 3
Weight
24.145kDa
Superfamily
protease inhibitor I35 (TIMP) family
Alternative Names
HSMRK222; K222; K222TA2; metalloproteinase inhibitor 3; MIG-5 protein; Protein MIG-5; pseudoinflammatory); SFD; TIMP metallopeptidase inhibitor 3; TIMP3; TIMP-3; tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy; Tissue inhibitor of metalloproteinases 3 TIMP3 HSMRK222, K222, K222TA2, SFD TIMP metallopeptidase inhibitor 3 metalloproteinase inhibitor 3|MIG-5 protein|TIMP-3|protein MIG-5|tissue inhibitor of metalloproteinases 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TIMP3, check out the TIMP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TIMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TIMP3 PicoKine® Quick ELISA Kit (FEK0523)
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