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Product Info Summary
| SKU: | LS003594 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of interleukin 12 receptor, beta 1 (IL12RB1), transcript variant 1 |
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Product info
Product Name
IL12RB1 (NM_005535) Human Over-expression Lysate
See all IL-12 R beta 1 products
SKU/Catalog Number
LS003594
Size
100 µg
Description
Transient overexpression lysate of interleukin 12 receptor, beta 1 (IL12RB1), transcript variant 1
Storage & Handling
Cite This Product
IL12RB1 (NM_005535) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003594)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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IL12RB1 Human Over-expression Lysates NM_005535.
Western blot validation of overexpression lysate (LS003594) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IL12RB1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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IL12RB1 Human Over-expression Lysates NM_153701.
Western blot validation of overexpression lysate (LS003594) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IL12RB1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for IL12RB1 (Source: Uniprot.org, NCBI)
Gene Name
IL12RB1
Full Name
Interleukin-12 receptor subunit beta-1
Weight
73.109kDa
Superfamily
type I cytokine receptor family
Alternative Names
CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454 IL12RB1 CD212, IL-12R-BETA1, IL12RB, IMD30 interleukin 12 receptor subunit beta 1 interleukin-12 receptor subunit beta-1|IL-12 receptor beta component|IL-12 receptor subunit beta-1|IL-12R subunit beta-1|cluster of differentiation 212|interleukin 12 receptor, beta 1|interleukin-12 receptor beta-1 chain
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IL12RB1, check out the IL12RB1 Infographic
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In this infographic, you will see the following information for IL12RB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IL12RB1 (NM_005535) Human Over-expression Lysate (LS003594)
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