Product Info Summary
SKU: | LS003614 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of IMP (inosine monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 1 |
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Product info
Product Name
IMPDH1 (NM_000883) Human Over-expression Lysate
SKU/Catalog Number
LS003614
Size
100 µg
Description
Transient overexpression lysate of IMP (inosine monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 1
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
IMPDH1 (NM_000883) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003614)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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IMPDH1 Human Over-expression Lysates NM_000883.
Western blot validation of overexpression lysate (LS003614) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IMPDH1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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IMPDH1 Human Over-expression Lysates NM_001102605.
Western blot validation of overexpression lysate (LS003614) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IMPDH1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
IMPDH1 Human Over-expression Lysates NM_001142575.
Western blot validation of overexpression lysate (LS003614) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IMPDH1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
IMPDH1 Human Over-expression Lysates NM_001142576.
Western blot validation of overexpression lysate (LS003614) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IMPDH1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
IMPDH1 Human Over-expression Lysates NM_183243.
Western blot validation of overexpression lysate (LS003614) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IMPDH1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for IMPDH1 (Source: Uniprot.org, NCBI)
Gene Name
IMPDH1
Full Name
Inosine-5'-monophosphate dehydrogenase 1
Weight
55.406kDa
Superfamily
IMPDH/GMPR family
Alternative Names
DKFZp781N0678; EC 1.1.1.205; IMP (inosine 5'-monophosphate) dehydrogenase 1; IMP (inosine monophosphate) dehydrogenase 1; IMP Dehydrogenase 1; IMPD 1; IMPD; IMPD1; IMPDH 1; IMPDH1; IMPDH-I; inosine-5'-monophosphate dehydrogenase 1; LCA11; LCA11RP10; retinitis pigmentosa 10 (autosomal dominant); RP10; sWSS2608 IMPDH1 IMPD, IMPD1, IMPDH-I, LCA11, RP10, sWSS2608 inosine monophosphate dehydrogenase 1 inosine-5-monophosphate dehydrogenase 1|IMP (inosine 5-monophosphate) dehydrogenase 1|IMP (inosine monophosphate) dehydrogenase 1|IMPD 1|IMPDH 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IMPDH1, check out the IMPDH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IMPDH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IMPDH1 (NM_000883) Human Over-expression Lysate (LS003614)
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