KMT5A (NM_020382) Human Over-expression Lysate

Product Name

KMT5A (NM_020382) Human Over-expression Lysate

See all SETD8 products

SKU/Catalog Number

LS387893

Size

100 µg

Description

Transient overexpression lysate of SET domain containing (lysine methyltransferase) 8 (SETD8)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

KMT5A (NM_020382) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS387893)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for KMT5A (Source: Uniprot.org, NCBI)

Alternative Names

H4-K20-HMTase SETD8; H4K20-specific histone methyltransferase splice variant Set8b; Histone-lysine N-methyltransferase SETD8; KMT5A SET domain-containing protein 8; KMT5A; Lysine N-methyltransferase 5A; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; PRSET7; PR-Set7EC 2.1.1.43; SET domain containing (lysine methyltransferase) 8; SET07SET8H4-K20-specific histone methyltransferase; SET8 Kmt5a|2410195B05Rik, AA617402, AW536475, PR-S, PR-SET7, PR/SET07, Setd, Setd8|lysine methyltransferase 5A|N-lysine methyltransferase KMT5A|H4-K20-HMTase KMT5A|H4-K20-HMTase SETD8|N-lysine methyltransferase SETD8|PR/SET domain-containing protein 07|SET domain containing (lysine methyltransferase) 8|SET domain-containing protein 8|histone-lysine N-methyltransferase KMT5A|histone-lysine N-methyltransferase SETD8|lysine-specific methylase 5A

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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