MASTL Colorimetric Cell-Based ELISA

MASTL ELISA kit, cell-based

The MASTL Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MASTL protein expression profile in cells. The kit can be used for measuring the relative amounts of MASTL in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MASTL.

Product Info Summary

SKU: EKC1899
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

Product Name

MASTL Colorimetric Cell-Based ELISA

See all MASTL products

SKU/Catalog Number

EKC1899

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The MASTL Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MASTL protein expression profile in cells. The kit can be used for measuring the relative amounts of MASTL in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MASTL.

Storage & Handling

4°C/6 Months

Cite This Product

MASTL Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1899)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1899 is reactive to MASTL in Human, Mouse samples

Application Guarantee

EKC1899 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-MASTL Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For MASTL (Source: Uniprot.Org, NCBI)

Uniprot ID

Q96GX5

Gene ID

84930

Gene Name

MASTL

Full Name

Serine/threonine-protein kinase greatwall

Weight

97.319kDa

Superfamily

protein kinase superfamily

Alternative Names

FLJ14813EC 2.7.11.1; Greatwall; GW; GWL; hGWL; MASTL; MAST-L; microtubule associated serine/threonine kinase-like; Microtubule-associated serine/threonine-protein kinase-like; RP11-85G18.2; THC2; THC2greatwall protein kinase MASTL GREATWALL, GW, GWL, MAST-L, THC2 microtubule associated serine/threonine kinase like serine/threonine-protein kinase greatwall|greatwall kinase homolog|greatwall protein kinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MASTL, check out the MASTL Infographic

MASTL infographic

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In this infographic you will see the following information for MASTL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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