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Product Info Summary
|Description:||Transient overexpression lysate of chemokine (C-C motif) ligand 2 (CCL2)|
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MCP1 (CCL2) (NM_002982) Human Over-expression Lysate
Transient overexpression lysate of chemokine (C-C motif) ligand 2 (CCL2)
Storage & Handling
Cite This Product
MCP1 (CCL2) (NM_002982) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006347)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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MCP1 (CCL2) Human Over-expression Lysates NM_002982.
Western blot validation of overexpression lysate (LS006347) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CCL2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for CCL2 (Source: Uniprot.org, NCBI)
C-C motif chemokine 2
intercrine beta (chemokine CC) family
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF CCL2 GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF C-C motif chemokine ligand 2 C-C motif chemokine 2|chemokine (C-C motif) ligand 2|monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte chemotactic protein 1|monocyte secretory protein JE|small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)|small inducible cytokine subfamily A (Cys-Cys), member 2|small-inducible cytokine A2*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on CCL2, check out the CCL2 Infographic
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