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Product Info Summary
| SKU: | EK1352 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse ANGPTL3 ELISA Kit PicoKine®
View all Angiopoietin-like Protein 3/ANGPTL3 ELISA kits
SKU/Catalog Number
EK1352
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse ANGPTL3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Angptl3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse ANGPTL3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1352)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S17-T455
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1352 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1352 is reactive to ANGPTL3 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1352 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Angiopoietin-like Protein 3/ANGPTL3
ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1352-CAP | Anti-Mouse ANGPTL3 Pre-coated 96-well strip microplate | 1 |
| EK1352-ST | Mouse ANGPTL3 Standard | 2 vials, 10 ng/tube |
| EK1352-DA | Mouse ANGPTL3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.006 | 0.064 | 0.125 | 0.259 | 0.511 | 0.936 | 1.623 | 2.094 |
Data Example Images
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Mouse ANGPTL3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Angiopoietin-like Protein 3/ANGPTL3, Dilution ratio of 1:50, concentration in serum and plasma is 14-39 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse ANGPTL3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 234 | 1410 | 5833 | 215 | 1499 | 6259 |
| Standard deviation | 14.7%1 | 59.22 | 274.15 | 17.2 | 79.44 | 337.98 |
| CV (%) | 6.8% | 4.2% | 4.7% | 8% | 5.3% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-like Protein 3/ANGPTL3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 234 | 260 | 243 | 235 | 243 | 10.41 | 4.2% |
| Sample 2 | 1410 | 1366 | 1348 | 1447 | 1392 | 38.59 | 2.7% |
| Sample 3 | 5833 | 5711 | 5318 | 5602 | 5616 | 190.46 | 3.3% |
Protein Target Info & Infographic
Gene/Protein Information For ANGPTL3 (Source: Uniprot.Org, NCBI)
Gene Name
ANGPTL3
Full Name
Angiopoietin-related protein 3
Weight
53.637kDa
Alternative Names
AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2 ANGPTL3 ANG-5, ANGPT5, ANL3, FHBL2 angiopoietin like 3 angiopoietin-related protein 3|angiopoietin 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ANGPTL3, check out the ANGPTL3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ANGPTL3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse ANGPTL3 ELISA Kit PicoKine® (EK1352)
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Customer Q&As
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8 Customer Q&As for Mouse ANGPTL3 ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
G. Hernandez
Verified customer
Asked: 2019-10-31
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-10-31
Question
Q: What is the optimal O.D. value for ANGPTL3 ELISA kit? I used your ANGPTL3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ANGPTL3 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-04-20
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ANGPTL3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of ANGPTL3 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-04-20
Question
Q: if the enzyme conjugated ANGPTL3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ANGPTL3 antigen?
Verified Customer
Verified customer
Asked: 2019-01-12
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-01-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
K. Chen
Verified customer
Asked: 2018-11-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-26
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
J. Garcia
Verified customer
Asked: 2018-08-09
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-08-09
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of ANGPTL3 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-06-17
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the ANGPTL3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-06-17
Question
Q: how to analyze ELISA data? I have obtained ANGPTL3 level in plasma.
P. Liu
Verified customer
Asked: 2018-02-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-02-09
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement ANGPTL3 using the EK1352 Mouse ANGPTL3 Picokine® ELISA Kit?
D. Dhar
Verified customer
Asked: 2017-08-19
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2017-08-19
