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Product Info Summary
| SKU: | EK1305 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine®
View all Siglec-1/CD169 ELISA kits
SKU/Catalog Number
EK1305
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CD169/SIGLEC-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Siglec1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1305)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: T20-R1639
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1305 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1305 is reactive to SIGLEC1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1305 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Siglec-1/CD169
SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1305-CAP | Anti-Mouse SIGLEC1 Pre-coated 96-well strip microplate | 1 |
| EK1305-ST | Mouse SIGLEC1 Standard | 2 vials, 10 ng/tube |
| EK1305-DA | Mouse SIGLEC1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.011 | 0.186 | 0.334 | 0.544 | 0.973 | 1.571 | 2.031 | 2.394 |
Data Example Images
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Mouse CD169/SIGLEC-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Siglec-1/CD169, Dilution ratio of 1:10, concentration in serum and plasma is around 7-16 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 331 | 1301 | 5540 | 351 | 1258 | 5216 |
| Standard deviation | 21.18 | 84.56 | 371.18 | 26.67 | 86.8 | 432.92 |
| CV (%) | 6.4% | 6.5% | 6.7% | 7.6% | 6.9% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Siglec-1/CD169 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 331 | 367 | 348 | 351 | 349 | 12.77 | 3.6% |
| Sample 2 | 1301 | 1260 | 1214 | 1306 | 1270 | 37.05 | 2.9% |
| Sample 3 | 5540 | 5164 | 5135 | 4886 | 5181 | 233.61 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For SIGLEC1 (Source: Uniprot.Org, NCBI)
Gene Name
SIGLEC1
Full Name
Sialoadhesin
Weight
182.624kDa
Superfamily
immunoglobulin superfamily
Alternative Names
CD169; FLJ00051; sialic acid binding Ig-like lectin 1, sialoadhesin; sialoadhesin; Siglec1; Siglec-1 SIGLEC1 CD169, SIGLEC-1, SN sialic acid binding Ig like lectin 1 sialoadhesin|sialic acid binding Ig-like lectin 1, sialoadhesin|sialic acid-binding immunoglobulin-like lectin 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SIGLEC1, check out the SIGLEC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SIGLEC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine® (EK1305)
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Customer Q&As
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5 Customer Q&As for Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Mouse CD169 Picokine® ELISA Kit (Catalog # EK1305)?
Verified Customer
Verified customer
Asked: 2020-05-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD169 causing degradation of its protein structure. CD169 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD169 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-05-22
Question
Q: which procedure should I follow in order to thaw whole blood sample for CD169 ELISA after freezing?
Y. Bhatt
Verified customer
Asked: 2020-05-07
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD169 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-05-07
Question
Q: What is the optimal O.D. value for CD169 ELISA kit? I used your CD169 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD169 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD169 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD169 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-01-29
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-11-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-11-09
Question
Q: if the enzyme conjugated CD169 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD169 antigen?
W. Martin
Verified customer
Asked: 2019-10-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-10-09
