Click image to see more details
Product Info Summary
| SKU: | EK1376 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Endostatin ELISA Kit PicoKine®
View all Endostatin ELISA kits
SKU/Catalog Number
EK1376
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Endostatin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Col18a1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Endostatin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1376)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: H1591-K1774
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1376 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1376 is reactive to Col18a1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1376 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Endostatin
Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1376-CAP | Anti-Mouse Col18a1 Pre-coated 96-well strip microplate | 1 |
| EK1376-ST | Mouse Col18a1 Standard | 2 vials, 10 ng/tube |
| EK1376-DA | Mouse Col18a1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.040 | 0.137 | 0.259 | 0.473 | 0.807 | 1.307 | 1.683 | 2.010 |
Data Example Images
Click image to see more details
Mouse Endostatin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Endostatin, Dilution ratio of 1:50, concentration in serum and plasma is around 50 ng/ml..
Some articles we found to cite concentrations of Endostatin in samples: 22322847, 19595115 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Endostatin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 306 | 1512 | 4729 | 308 | 1404 | 4765 |
| Standard deviation | 12.24 | 74.08 | 316.84 | 13.86 | 77.22 | 390.73 |
| CV (%) | 4% | 4.9% | 6.7% | 4.5% | 5.5% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Endostatin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 306 | 291 | 279 | 313 | 297 | 13.19 | 4.4% |
| Sample 2 | 1512 | 1379 | 1586 | 1539 | 1504 | 76.87 | 5.1% |
| Sample 3 | 4729 | 3933 | 4390 | 4218 | 4317 | 288.23 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For Col18a1 (Source: Uniprot.Org, NCBI)
Gene Name
Col18a1
Full Name
Collagen alpha-1(XVIII) chain
Weight
182.172kDa
Superfamily
multiplexin collagen family
Alternative Names
antiangiogenic agent; COL18A1; collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; Endostatin; FLJ27325; FLJ34914; human type XVIII collagen10endostatin; KNO; KNO1MGC74745; Knobloch syndrome, type 1; KS; multi-functional protein MFP Col18a1|endo|collagen, type XVIII, alpha 1|collagen alpha-1(XVIII) chain|alpha-1(XVIII) collagen|endostatin|procollagen, type XVIII, alpha 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Col18a1, check out the Col18a1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Col18a1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Endostatin ELISA Kit PicoKine® (EK1376)
Hello CJ!
EK1376 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
IL-22-producing Th22 cells play a protective role in CVB3-induced chronic myocarditis and dilated cardiomyopathy by inhibiting myocardial fibrosis
IL-22-producing Th22 cells play a protective role in CVB3-induced chronic myocarditis and dilated cardiomyopathy by inhibiting myocardial fibrosis
Zhang Y, Qu Zh, Cui M, Guo C, Zhang Xm, Ma Ch, Sun Ws. Cancer Biol Ther. 2009 Mar;8(5):466-73. Epub 2009 Mar 21. Combined Endostatin And Trail Gene Transfer Suppresses Human Hepatocellular Carcinoma Growth And Angiogenesis In Nude Mice.
Fu Y, Zhao Z, Wu Y, Wu K, Xu X, Liu Y, Tong C. J Ethnopharmacol. 2012 Jan 6;139(1):26-33. Doi: 10.1016/J.Jep.2011.08.077. Epub 2011 Nov 10. Therapeutic Mechanisms Of Tongmai Dasheng Tablet On Tripterygium Glycosides Induced Rat Model For Premature...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Mouse Endostatin ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Mouse Endostatin ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
11 Customer Q&As for Mouse Endostatin ELISA Kit PicoKine®
Question
Q: Can Endostatin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
D. Singh
Verified customer
Asked: 2020-08-30
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-08-30
Question
Q: how to proceed with the analysis of ELISA data? I have performed Endostatin level in plasma.
Verified Customer
Verified customer
Asked: 2020-06-24
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-06-24
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
L. Turner
Verified customer
Asked: 2020-02-19
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-19
Question
We have a customer who is interrested in your Mouse endostatin ELISA kit and have some questions: Does it also detect collagenXVIII (I am running the assay on the brain lysates that most likely contain both endostatin and unprocessed collagenXVIII)? You recommend using heparin or EDTA-coated vials for plasma collection. Can also citrate-coated vials be used? Or the assay would not work on citrate-plasma?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
1.We haven't had
Boster Scientific Support
Answered: 2019-12-23
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-08-08
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Endostatin using the EK1376 Mouse Endostatin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-05-30
Question
Q: which procedure should I follow in order to thaw whole blood sample for Endostatin ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Endostatin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-05-29
Question
Could you confirm what was used to raise the antibodies of EK1376? Was it a peptide or was it the full recombinant protein?
Verified customer
Asked: 2019-04-04
Answer
A recombinant protein (immunogen sequence: H1591-K1774) was used to raise the antibodies of the Mouse Endostatin ELISA Kit PicoKine™ (EK1376).
Boster Scientific Support
Answered: 2019-04-08
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-07-01
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-07-01
Question
Q: What is the optimal O.D. value for Endostatin ELISA kit? I performed your Endostatin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Endostatin even though the O.D. values are not very high?
L. Robinson
Verified customer
Asked: 2016-02-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Endostatin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Endostatin that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2016-02-15
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2014-09-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-09-12
