Mouse FGF21 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

SKU:EZ1379
Pack Size:For 5 plates, 96 wells each
Validated Species:Mouse
Application:ELISA
Sensitivity:<10pg/ml
Assay Range:31.2pg/ml-2000pg/ml
Sample Type:cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
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Product Features:

Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

Overview

Product Name Mouse FGF21 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
SKU/Catalog Number EZ1379
Description A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects FGF21 in Mouse samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
Cite This Product Mouse FGF21 EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1379)
Replacement Item This antibody may replace the following items: from Santa Cruz Biotechnology.
Host
Validated Species 193
Predicted Species

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application 18

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen Expression system for standard: E.coli, A29-S210
Conjugate
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Pack Size For 5 plates, 96 wells each

Properties

Clonality
Clone Number
Sensitivity <10pg/ml
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
Assay Range 31.2pg/ml-2000pg/ml
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Capture Antibody
Detection Antibody
Normal Levels Some research articles suggesting the natural levels of FGF21 are listed below:
Form
Contents
Concentration
Activating Reagent
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Purification
Isotype

Kit Components

Catalog numberDescriptionQuantityBuffersEZ1379-CARabbit anti- mouse FGF21 polyclonal antibody(Capture Antibody)500 μL, 0.4mg/mL(recommended dilution 1:100)0.5% Na2HPO4, 0.02% Sodium azide and 50% GlycerolEZ1379-DABiotinylated goat anti- mouse FGF21 polyclonal antibody(Detection Antibody)500 μL, 2.1μg/mL(recommended dilution 1:100)0.02% Sodium azide, 0.5%BSA, 0.2%Tris, 1%NaCl and 30% GlycerolEZ1379-STLyophilized recombinant mouse FGF21 standard2ng/tubex2AR1103Avidin-Biotin-Peroxidase Complex(ABC)500ul(recommended dilution 1:100)<

*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.

See Prices For Extra Components

Material Required But Not Provided

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers
  • 7. Capture Antibody Diluent: 100 mL H2O, 0.5 g Na2HPO4.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 ?m filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (neutral PBS or TBS).
    Preparation of 0.01M TBS: Add 1.2 g Tris, 8.5 g Nacl; 450 ?L of purified acetic acid or 700 ?L of concentrated hydrochloric acid to 1000 mL H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1 L.
    Preparation of 0.01M PBS: Add 8.5 g sodium chloride, 1.4 g Na2HPO4 and 0.2 g NaH2PO4 to 1000 mL distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*The EZ Set Accessory Kit (EZA001) provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you.

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name FGF21
Protein Name
Molecular Weight
Protein Function Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB.
Tissue Specificity Most abundantly expressed in the liver, also expressed in the thymus at lower levels.
Sequence Similarities
Subcellular Localization Secreted
Uniprot ID Q9JJN1
Alternative Names Fibroblast growth factor 21; FGF-21; Fgf21;
Reseach Areas 2433,2329,2338,2435,2393,2320|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for FGF21

Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

Mouse FGF21 EZ-Set™ ELISA Kit (DIY Antibody Pairs) Images

Click the images to enlarge.

Mouse FGF21 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
Mouse FGF21 EZ-Set ELISA Kit standard curve

Intra/Inter Assay Precision

|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|64|320|918|65|289|938 Standard deviation|2.81|24|52.32|3.12|27.16|70.35 CV(%)|4.4%|7.5%|5.7%|4.8%|9.4%|7.5%

Typical Data Obtained from Mouse FGF21 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

(TMB reaction incubate at 37℃ for 25-30min)

Concentration(pg/ml)031.262.512525050010002000
O.D.0.0190.0930.1840.3220.5620.9961.6522.395

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects FGF21 in Mouse samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
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EZ1379
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