Mouse GM-CSF PicoKine™ Quick ELISA Kit

Mouse GM-CSF PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Csf2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Product Info Summary

SKU: FEK0365
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 90 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Mouse GM-CSF PicoKine™ Quick ELISA Kit

See all GM-CSF products

SKU/Catalog Number

FEK0365

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse GM-CSF PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Csf2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse GM-CSF PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0365)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A18-K141

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0365 is reactive to Csf2 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0365 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity
FEK0365-CAP 96-well plate precoated with anti-Mouse GM-CSF antibody 1
FEK0365-ST lyophilized recombinant Mouse GM-CSF standard 10 ng/tube
FEK0365-DA Biotinylated anti-Mouse GM-CSF detection antibody (50x) 130 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0070.0410.0980.1720.4650.7601.5022.104

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3223448035213448
Standard deviation1.3417%819.681.5414.9121.05
CV (%)4.2%7%4.1%4.4%7%4.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GM-CSF in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 132363832342.597.6%
Sample 22342402222232297.563.3%
Sample 348044348748047217.263.6%
*number of samples for each test n=16.

Gene/Protein Information For Csf2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P01587

Gene ID

12981

Gene Name

Csf2

Full Name

Granulocyte-macrophage colony-stimulating factor

Weight

16.091kDa

Superfamily

GM-CSF family

Alternative Names

colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim Csf2|CSF, Csfg, Csfgm, GMCS, GMCSF, Gm-CS, Gm-CSf, MGI-I, MGI-IGM|colony stimulating factor 2 (granulocyte-macrophage)|granulocyte-macrophage colony-stimulating factor|colony-stimulating factor|granulocyte-macrophage colony stimulating factor 2|put. GM-CSF

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Csf2, check out the Csf2 Infographic

Csf2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Csf2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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