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Product Info Summary
| SKU: | EK0381 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse IGF-2 ELISA Kit PicoKine®
View all IGF-II/IGF2 ELISA kits
SKU/Catalog Number
EK0381
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IGF-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Igf2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IGF-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0381)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A25-E91
Sensitivity
<5 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0381 for more details
Cross-reactivity
There is no detectable cross-reactivity with IGF-1.
Reactive Species
EK0381 is reactive to IGF2 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0381 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGF-II/IGF2
Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0381-CAP | Anti-Mouse IGF2 Pre-coated 96-well strip microplate | 1 |
| EK0381-ST | Mouse IGF2 Standard | 2 vials, 10 ng/tube |
| EK0381-DA | Mouse IGF2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.091 | 0.146 | 0.179 | 0.275 | 0.507 | 0.789 | 1.143 | 1.990 |
Data Example Images
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Mouse IGF-2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGF-II/IGF2, Dilution ratio of 1:1, concentration in serum and plasma is 400-1000 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IGF-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 83 | 719 | 980 | 84 | 692 | 946 |
| Standard deviation | 5.89 | 56.08 | 61.74 | 6.55 | 63.66 | 73.78 |
| CV (%) | 7.1% | 7.8% | 9.2%% | 7.8% | 9.2% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IGF-II/IGF2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 83 | 84 | 91 | 92 | 87 | 4.03 | 4.6% |
| Sample 2 | 719 | 780 | 698 | 710 | 726 | 31.63 | 4.3% |
| Sample 3 | 980 | 989 | 1144 | 1081 | 1048 | 67.83 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For IGF2 (Source: Uniprot.Org, NCBI)
Gene Name
IGF2
Full Name
Insulin-like growth factor II
Weight
20.14kDa
Superfamily
insulin family
Alternative Names
C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A IGF2 C11orf43, GRDF, IGF-II, PP9974, SRS3 insulin like growth factor 2 insulin-like growth factor II|T3M-11-derived growth factor|insulin-like growth factor 2 (somatomedin A)|insulin-like growth factor type 2|preptin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IGF2, check out the IGF2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IGF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IGF-2 ELISA Kit PicoKine® (EK0381)
Hello CJ!
EK0381 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
IMP2 Increases Mouse Skeletal Muscle Mass and Voluntary Activity by Enhancing Autocrine Insulin-Like Growth Factor 2 Production and Optimizing Muscle Metabolism
Decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging
RNA m6A reader IMP2/IGF2BP2 promotes pancreatic β-cell proliferation and insulin secretion by enhancing PDX1 expression
Elevated insulin-like growth factor 2 expression may contribute to the hypermuscular phenotype of myostatin null mice
Regué L,Zhao L,Ji F,Wang H,Avruch J,Dai N. RNA m6A reader IMP2/IGF2BP2 promotes pancreatic β-cell proliferation and insulin secretion by enhancing PDX1 expression. Mol Metab. 2021 Mar 8:101209.doi:10.1016/j.molmet.2021.101209.Epub ahead of print.PMID:33705986.
Species: Human,Mouse
EK0381 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Elevated insulin-like growth factor 2 expression may contribute to the hypermuscular phenotype of myostatin null mice.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
11 Customer Q&As for Mouse IGF-2 ELISA Kit PicoKine®
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
P. Liu
Verified customer
Asked: 2020-12-07
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-12-07
Question
Q: how can I thaw whole blood sample for IGF-II ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-06-16
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGF-II for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-06-16
Question
Q: how to proceed with the analysis of ELISA data? I have obtained IGF-II level in cell lysates.
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-02-05
Question
Q: if the enzyme conjugated IGF-II antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IGF-II antigen?
S. Scott
Verified customer
Asked: 2019-11-15
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-11-15
Question
Q: can I use citrate plasma as samples in Mouse IGF-II Picokine® ELISA Kit (Catalog # EK0381)?
W. Liu
Verified customer
Asked: 2019-11-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IGF-II causing disruption of its protein structure. IGF-II may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IGF-II ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-11-07
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
B. Mangal
Verified customer
Asked: 2019-09-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-09-23
Question
Is EK0381 suitable for use with cerebrospinal fluid?
Verified Customer
Verified customer
Asked: 2019-09-15
Answer
Unfortunately, we haven't tested the Mouse IGF-2 ELISA Kit PicoKine™ (EK0381) on cerebrospinal fluid. We are uncertain if the kits are able to work on cerebrospinal fluid. Please run a pilot test if you want to try. In theory, if the expression level of the targets protein in the cerebrospinal fluid falls in the assay range of the kits, the kits are able to detect the protein in the sample.
Boster Scientific Support
Answered: 2019-09-16
Question
Q: What is the optimal O.D. value for IGF-II ELISA kit? I used your IGF-II ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IGF-II even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IGF-II ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of IGF-II that can be declared/interpreted as positive by the above standard is 5pg/ml.
Boster Scientific Support
Answered: 2019-06-14
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-12-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-30
Question
Dear Boster Support: In regards to your EK0381 Mouse IGF-2 ELISA Kit, I would like to know if the primary anti-IGF2 antibody that you use in your EK0381 Mouse IGF-2 ELISA Kit is specific to mouse IGF2? Does it have any cross-reactivity? Either with mouse or human IGF1? Do you have an idea at what region of the IGF2 protein the antibody binds?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
Regarding the specificity information about EK0381 Mouse IGF-2 ELISA Kit stated as follows: Capture antibody; Detects mouse IGF
Boster Scientific Support
Answered: 2018-07-19
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IGF-II using the EK0381 Mouse IGF-II Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2014-12-17
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2014-12-17
