Product Info Summary
SKU: | EZ0417 |
---|---|
Size: | For 5 plates, 96 wells each |
Reactive Species: | Mouse |
Application: | ELISA |
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Product info
Product Name
Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
SKU/Catalog Number
EZ0417
Size
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse IL10 in cell culture supernatants, serum.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0417)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S19-N178
Assay Range
15.6 pg/ml - 1,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ0417 is reactive to IL10 in Mouse samples
Validated Sample Types
cell culture supernatants, serum.
Application Guarantee
EZ0417 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ0417-CA | Rabbit anti- mouse Il10 polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ0417-DA | Biotinylated goat anti- mouse Il10 polyclonal antibody (Detection Antibody) | 500 µl, 14 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK0417-ST | Lyophilized recombinant mouse Il10 standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.055 | 0.129 | 0.184 | 0.322 | 0.507 | 0.943 | 1.294 | 1.905 |
Data Example Images
Click image to see more details
Mouse IL-10 EZ-Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 33 | 219 | 466 | 35 | 203 | 420 |
Standard deviation | 1.88 | 14.01 | 19.1 | 2.66 | 16.64 | 25.2 |
CV (%) | 5.7% | 6.4% | 4.1% | 7.6% | 8.2% | 6% |
Protein Target Info & Infographic
Gene/Protein Information For IL10 (Source: Uniprot.Org, NCBI)
Gene Name
IL10
Full Name
Interleukin-10
Weight
20.517kDa
Superfamily
IL-10 family
Alternative Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL10, check out the IL10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0417)
Hello CJ!
EZ0417 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
TLR4 signaling pathway in mouse Lewis lung cancer cells promotes the expression of TGF-β1 and IL-10 and tumor cells migration
MiR-137 attenuates spinal cord injury by modulating NEUROD4 through reducing inflammation and oxidative stress
Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis
Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica
Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells
Lu C, Liu H, Jin X, Chen Y, Liang CL, Qiu F, Dai Z. Front Pharmacol. 2018 Feb 12;9:88. doi: 10.3389/fphar.2018.00088. eCollection 2018. Herbal Components of a Novel Formula PSORI-CM02 Interdependently Suppress Allograft Rejection and Induce CD8+CD...
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
Question
What is the clone number of EZ0417 antibody that the plates are coated with?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The EZ0417 antibody is a goat polyclonal as capture antibody, and a rat monoclonal [B-C40] for detection.
Boster Scientific Support
Answered: 2020-01-17
Question
I would like to ask if this Mouse IL-10 ELISA Kit EZ-Set
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
The antibody Mouse IL-10 ELISA Kit EZ-Set
Boster Scientific Support
Answered: 2018-06-25