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Product Info Summary
| SKU: | EK0714 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Mouse IL7R/CD127 ELISA Kit PicoKine®
View all IL-7R alpha/CD127 ELISA kits
SKU/Catalog Number
EK0714
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL7R/CD127 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il7r in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL7R/CD127 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0714)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E21-D239
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0714 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0714 is reactive to IL7R in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0714 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-7R alpha/CD127
The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0714-CAP | Anti-Mouse IL7R Pre-coated 96-well strip microplate | 1 |
| EK0714-ST | Mouse IL7R Standard | 2 vials, 20 ng/tube |
| EK0714-DA | Mouse IL7R Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.060 | 0.135 | 0.214 | 0.338 | 0.568 | 0.893 | 1.461 | 1.912 |
Data Example Images
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Mouse IL7R/CD127 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-7R alpha/CD127, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 31.2 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL7R/CD127 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 66 | 270 | 964 | 61 | 293 | 970 |
| Standard deviation | 2.77 | 18.09 | 74.22 | 3.53 | 22.26 | 82.45 |
| CV (%) | 4.2% | 6.7% | 7.7% | 5.8% | 7.6% | 8.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-7R alpha/CD127 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 66 | 77 | 65 | 68 | 69 | 4.74 | 6.8% |
| Sample 2 | 270 | 257 | 287 | 288 | 275 | 12.85 | 4.6% |
| Sample 3 | 964 | 912 | 900 | 813 | 897 | 54.26 | 6% |
Protein Target Info & Infographic
Gene/Protein Information For IL7R (Source: Uniprot.Org, NCBI)
Gene Name
IL7R
Full Name
Interleukin-7 receptor subunit alpha
Weight
51.579kDa
Superfamily
type I cytokine receptor family
Alternative Names
CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha IL7R CD127, CDW127, IL-7R-alphaA, ILRA, IL7R interleukin 7 receptor interleukin-7 receptor subunit alpha|CD127 antigen|IL-7 receptor subunit alpha|IL-7R subunit alpha|interleukin 7 receptor alpha chain
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL7R, check out the IL7R Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL7R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL7R/CD127 ELISA Kit PicoKine® (EK0714)
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No publications found for EK0714
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Mouse IL7R/CD127 ELISA Kit PicoKine®
Question
Q: how to analyze ELISA data? I measured CD127 level in serum.
C. Banerjee
Verified customer
Asked: 2020-09-25
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-09-25
Question
For the kit EK0714 a potential customer asks for your in-house testing data with mouse serum samples. Would you have this information available to share?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
COA is sent.
Boster Scientific Support
Answered: 2020-04-16
Question
Have you tested EK0714 kit with mouse serum and can it be returned if it is not working with mouse serum upon purchase?
Verified customer
Asked: 2019-09-25
Answer
The Mouse IL7R/CD127 ELISA Kit PicoKine EK0714 has been validated for mouse cell culture supernates and serum samples. If the kit is not performing as expected for the validated species and applications listed on the product page, then our lab technicians will troubleshoot and if they find that there is a quality issue with the kit, then we will provide a replacement of another lot or a refund for the kit.
Boster Scientific Support
Answered: 2019-09-25
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-06-19
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CD127 using the EK0714 Mouse CD127 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-06-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Maharaj
Verified customer
Asked: 2018-06-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-06-10
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
T. Williams
Verified customer
Asked: 2018-05-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-05-24
Question
Q: if the enzyme conjugated CD127 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD127 antigen?
Verified Customer
Verified customer
Asked: 2016-05-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-05-23
