Click image to see more details
Product Info Summary
| SKU: | EK1274 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse LBP ELISA Kit PicoKine®
SKU/Catalog Number
EK1274
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse LBP ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Lbp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse LBP ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1274)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G25-V481
Sensitivity
<50 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1274 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1274 is reactive to LBP in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1274 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LBP
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1274-CAP | Anti-Mouse LBP Pre-coated 96-well strip microplate | 1 |
| EK1274-ST | Mouse LBP Standard | 2 vials, 100 ng/tube |
| EK1274-DA | Mouse LBP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.038 | 0.331 | 0.445 | 0.553 | 0.974 | 1.140 | 1.528 | 1.914 |
Data Example Images
Click image to see more details
Mouse LBP PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect LBP, Dilution ratio of 1:100, concentration in serum and plasma is around 500 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse LBP ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 3313 | 12997 | 39089 | 3385 | 14212 | 39495 |
| Standard deviation | 237.12 | 831.8 | 2110.8 | 243.72 | 1151.17 | 2804.14 |
| CV (%) | 7.1% | 6.4% | 5.4% | 7.2% | 8.1% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of LBP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 3313 | 3708 | 3123 | 3191 | 3333 | 226.54 | 6.7% |
| Sample 2 | 12997 | 12936 | 11430 | 12001 | 12341 | 657.62 | 5.3% |
| Sample 3 | 39089 | 41588 | 44126 | 37423 | 40556 | 2538.58 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For LBP (Source: Uniprot.Org, NCBI)
Gene Name
LBP
Full Name
Lipopolysaccharide-binding protein
Weight
53.384kDa
Superfamily
BPI/LBP/Plunc superfamily
Alternative Names
LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; LPS-binding protein; MGC22233 LBP BPIFD2 lipopolysaccharide binding protein lipopolysaccharide-binding protein|BPI fold containing family D, member 2|LPS-binding protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LBP, check out the LBP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LBP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse LBP ELISA Kit PicoKine® (EK1274)
Hello CJ!
EK1274 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Effect of Hops (Humulus lupulus L.) Extract Supplementation on Weight Gain, Adiposity and Intestinal Function in Ovariectomized Mice
Changes in Mouse Gut Microbial Community in Response to the Different Types of Commonly Consumed Meat
Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5
Prebiotic role of softwood hemicellulose in healthy mice model
Effect of Antarctic Krill Phospholipid (KOPL) on High Fat Diet-Induced Obesity in Mice
Modulation of high fat diet-induced microbiome changes, but not behaviour, by minocycline
Roles of the Conserved Amino Acid Residues in Reduced Human Defensin 5: Cysteine and Arginine Are Indispensable for Its Antibacterial Action and LPS Neutralization
Xingwei Xiang,Xiaoling Zhou,Wenjie Wang,Yufang Zhou,Xuxia Zhou,Shanggui Deng,Bin Zheng,Zhengshun Wen,Effect of Antarctic Krill Phospholipid (KOPL) on High Fat Diet-Induced Obesity in Mice,Food Research International,2021,110456,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110456.
Species: Mouse
EK1274 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Dorigatti JD,Thyne KM,Ginsburg BC,Salmon AB.Beta-guanidinopropionic acid has age-specific effects on markers of health and function in mice.Geroscience.2021 Apr 23.doi:10.1007/s11357-021-00372-8.Epub ahead of print.PMID:33890206.
Species: Mouse
EK1274 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Mouse LBP ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Mouse LBP ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Mouse LBP ELISA Kit PicoKine®
Question
Has EK1274 been tested for tissue extraction?
Verified customer
Asked: 2020-09-30
Answer
Our lab hasn't woked on tissue extraction using the Mouse LBP ELISA Kit PicoKine™ (EK1274). Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer to run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2020-10-08
Question
Q: how do I analyze ELISA data? I have performed LBP level in cell lysates.
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-02-03
Question
Q: What is the optimal O.D. value for LBP ELISA kit? I used your LBP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain LBP even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-01-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this LBP ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of LBP that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-01-05
Question
Q: how to thaw whole blood sample for LBP ELISA after freezing?
S. Harris
Verified customer
Asked: 2019-10-25
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test LBP for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-10-25
Question
Q: if the enzyme conjugated LBP antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the LBP antigen?
Verified Customer
Verified customer
Asked: 2019-07-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-07-21
Question
Q: Can LBP ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
D. Martinez
Verified customer
Asked: 2018-05-26
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-05-26
Question
What is the recommended dilution ratio for mouse serum samples?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
The recommended dilution ratio for mouse serum samples is from 1:400-800.
Boster Scientific Support
Answered: 2017-11-27
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-03-03
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-03-03
Question
Q: can I use citrate plasma as samples in Mouse LBP Picokine® ELISA Kit (Catalog # EK1274)?
Verified Customer
Verified customer
Asked: 2016-09-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of LBP causing degradation of its protein structure. LBP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the LBP ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-09-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-07-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-07-20
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
J. Lee
Verified customer
Asked: 2016-03-10
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-03-10
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2014-12-07
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-12-07
Question
Does the Mouse LBP ELISA kit have any cross reactivity to other proteins?
Verified Customer
Verified customer
Asked: 2014-09-05
Answer
Apolipoprotein A-1, BPI, TLR2, TLR4, CD14, MD-2 were tested and found no cross-reactivity.
Boster Scientific Support
Answered: 2014-09-05
