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Product Info Summary
| SKU: | EK0440 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Leptin Receptor ELISA Kit PicoKine®
SKU/Catalog Number
EK0440
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Leptin Receptor ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Lepr in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Leptin Receptor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0440)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L22-G839
Sensitivity
<5 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0440 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0440 is reactive to LEPR in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0440 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Leptin R
Leptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0440-CAP | Anti-Mouse LEPR Pre-coated 96-well strip microplate | 1 |
| EK0440-ST | Mouse LEPR Standard | 2 vials, 10 ng/tube |
| EK0440-DA | Mouse LEPR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.016 | 0.070 | 0.128 | 0.248 | 0.458 | 0.817 | 1.452 | 2.011 |
Data Example Images
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Mouse Leptin receptor PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Leptin Receptor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 222 | 1398 | 4930 | 200 | 1476 | 5345 |
| Standard deviation | 15.09 | 86.87 | 359.89 | 15.8 | 110.7 | 427.6 |
| CV (%) | 6.8% | 6.1% | 7.3% | 7.9% | 7.5% | 8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Leptin R in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 222 | 189 | 209 | 209 | 207 | 11.79 | 5.6% |
| Sample 2 | 1398 | 1396 | 1505 | 1381 | 1420 | 49.51 | 3.4% |
| Sample 3 | 4930 | 5654 | 5332 | 5633 | 5387 | 293.12 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For LEPR (Source: Uniprot.Org, NCBI)
Gene Name
LEPR
Full Name
Leptin receptor
Weight
132.494kDa
Superfamily
type I cytokine receptor family
Alternative Names
B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295 LEPR CD295, LEP-RD, OB-R, OBR, LEPR leptin receptor leptin receptor|OB receptor|huB219
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LEPR, check out the LEPR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LEPR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Leptin Receptor ELISA Kit PicoKine® (EK0440)
Hello CJ!
EK0440 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Titanium dioxide nanoparticle stimulating pro-inflammatory responses in vitro and in vivo for inhibited cancer metastasis
In utero exposure to di-(2-ethylhexyl) phthalate induces metabolic disorder and increases fat accumulation in visceral depots of C57BL/6J mice offspring
Exogenous leptin affects sperm parameters and impairs blood testis barrier integrity in adult male mice
The effect of intermittent hypoxia on bodyweight, serum glucose and cholesterol in obesity mice
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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14 Customer Q&As for Mouse Leptin Receptor ELISA Kit PicoKine®
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Leptin R in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-05-11
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Leptin R ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-05-11
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-12-12
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-12-10
Question
What is the capture and detection host for EK0440?
Verified customer
Asked: 2019-11-07
Answer
For the Mouse Leptin Receptor ELISA Kit PicoKine™ (EK0440), the capture host is Rat Monoclonal and detection host is Goat Polyclonal.
Boster Scientific Support
Answered: 2019-11-08
Question
What is the method of purification of the capture and detection host for EK0440?
Verified customer
Asked: 2019-11-07
Answer
For the Mouse Leptin Receptor ELISA Kit PicoKine™ (EK0440), the capture purification is Protein A or G purified and the detection purification is Affinity purified.
Boster Scientific Support
Answered: 2019-11-08
Question
What is the standard source and expression source of EK0440?
Verified customer
Asked: 2019-11-07
Answer
For the Mouse Leptin Receptor ELISA Kit PicoKine™ (EK0440), the standard source is Recombinant Protein and expression source is NSO Expression System.
Boster Scientific Support
Answered: 2019-11-08
Question
What is the immunogen for the capture antibody of EK0440?
Verified customer
Asked: 2019-11-07
Answer
The immunogen for the capture antibody of the Mouse Leptin Receptor ELISA Kit PicoKine™ (EK0440) is L22-G839.
Boster Scientific Support
Answered: 2019-11-08
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Z. Kulkarni
Verified customer
Asked: 2018-10-31
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-31
Question
I would like to ask if this EK0440 kit be used with blood samples. Thanks
Verified Customer
Verified customer
Asked: 2018-03-30
Answer
The product EK0440 kit have been tested and validated to use with plasma, serum and cell culture supernatant samples. However, if you are using whole blood samples we would not recommend it since it may increase a chance of experiencing high background and interference.
Boster Scientific Support
Answered: 2018-03-30
Question
Q: if the enzyme conjugated Leptin R antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Leptin R antigen?
Verified Customer
Verified customer
Asked: 2016-05-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-05-26
Question
Q: is it okay to use citrate plasma as samples in Mouse Leptin R Picokine® ELISA Kit (Catalog # EK0440)?
Verified Customer
Verified customer
Asked: 2016-05-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Leptin R causing degradation of its protein structure. Leptin R may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Leptin R ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-05-23
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Leptin R using the EK0440 Mouse Leptin R Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-09-06
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2015-09-06
Question
Q: how can I thaw whole blood sample for Leptin R ELISA after freezing?
Verified Customer
Verified customer
Asked: 2015-07-07
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Leptin R for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2015-07-07
Question
For this antibody EK0440; what type of graph is required for the results of this kit?
Verified Customer
Verified customer
Asked: 2014-04-08
Answer
The reading results for this EK0440 kit used a log-log model as this type of plot will fit most data sets very well. But you can use any graphing model including linear regression, 4-parameter and 2-order polynomial. During the testing of this kit we plots the ELISA data using log-log, linear regression, AND 4-parameter and then choose the model that fits the data best.
Boster Scientific Support
Answered: 2014-04-08
