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Product Info Summary
| SKU: | EK0811 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates and serum. |
Product info
Product Name
Mouse Mer ELISA Kit PicoKine®
SKU/Catalog Number
EK0811
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Mer ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mertk in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Mer ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0811)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E23-F498
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0811 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0811 is reactive to MERTK in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates and serum.
Application Guarantee
EK0811 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Mer
Proto-oncogene tyrosine-protein kinase MER, also called MERTK is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. This gene is mapped to 2q13. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. This gene also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0811-CAP | Anti-Mouse MERTK Pre-coated 96-well strip microplate | 1 |
| EK0811-ST | Mouse MERTK Standard | 2 vials, 10 ng/tube |
| EK0811-DA | Mouse MERTK Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.016 | 0.095 | 0.194 | 0.302 | 0.603 | 0.990 | 1.456 | 2.002 |
Data Example Images
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Mouse MER/MERTK PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Mer, Dilution ratio of 1:1, concentration in serum is 150-400 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Mer ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 109 | 571 | 2250 | 114 | 562 | 2147 |
| Standard deviation | 6.86 | 30.83 | 132.75 | 8.55 | 39.34 | 145.99 |
| CV (%) | 7% | 5.4% | 5.9% | 7.5% | 7% | 6.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Mer in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 109 | 127 | 129 | 123 | 122 | 7.81 | 6.4% |
| Sample 2 | 571 | 619 | 574 | 585 | 587 | 19.05 | 3.2% |
| Sample 3 | 2250 | 2045 | 2170 | 2458 | 2230 | 150.17 | 6.7% |
Protein Target Info & Infographic
Gene/Protein Information For MERTK (Source: Uniprot.Org, NCBI)
Gene Name
MERTK
Full Name
Tyrosine-protein kinase Mer
Weight
110.249kDa
Superfamily
protein kinase superfamily
Alternative Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer MERTK MER, RP38, Tyro12, c-Eyk, c-mer MER proto-oncogene, tyrosine kinase tyrosine-protein kinase Mer|MER receptor tyrosine kinase|STK kinase|c-mer proto-oncogene tyrosine kinase|proto-oncogene c-Mer|receptor tyrosine kinase MerTK
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MERTK, check out the MERTK Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MERTK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Mer ELISA Kit PicoKine® (EK0811)
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No publications found for EK0811
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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10 Customer Q&As for Mouse Mer ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for Mer ELISA kit? I used your Mer ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Mer even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-06-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Mer ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Mer that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-06-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-03-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-03-14
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Mer in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
S. Zhang
Verified customer
Asked: 2020-03-01
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Mer ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-01
Question
Q: how to thaw whole blood sample for Mer ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-02-01
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Mer for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-02-01
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Mer using the EK0811 Mouse Mer Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-03-05
Question
Q: how to proceed with the analysis of ELISA data? I have performed Mer level in serum.
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-08-22
Question
Q: if the enzyme conjugated Mer antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Mer antigen?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-07-20
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-06-05
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
K. Surve
Verified customer
Asked: 2018-06-03
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-06-03
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
C. Wilson
Verified customer
Asked: 2017-10-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-10-22
