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Product Info Summary
| SKU: | EK0495 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants. serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse Rantes ELISA Kit PicoKine®
View all CCL5/RANTES ELISA kits
SKU/Catalog Number
EK0495
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Rantes ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl5 in cell culture supernatants. serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Rantes ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0495)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S24-S91
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0495 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0495 is reactive to CCL5 in Mouse samples
Validated Sample Types
cell culture supernatants. serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0495 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL5/RANTES
Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0495-CAP | Anti-Mouse CCL5 Pre-coated 96-well strip microplate | 1 |
| EK0495-ST | Mouse CCL5 Standard | 2 vials, 10 ng/tube |
| EK0495-DA | Mouse CCL5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.006 | 0.085 | 0.189 | 0.402 | 0.797 | 1.014 | 1.579 | 2.038 |
Data Example Images
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Mouse Rantes PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL5/RANTES, Dilution ratio of 1:1, concentration in serum is around 30 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Rantes ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 29 | 191 | 416 | 26 | 175 | 442 |
| Standard deviation | 1.65 | 8.97 | 24.54 | 1.71 | 10.85 | 29.17 |
| CV (%) | 5.7% | 4.7% | 5.9% | 6.6% | 6.2% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL5/RANTES in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 32 | 28 | 28 | 29 | 1.63 | 5.6% |
| Sample 2 | 191 | 178 | 177 | 194 | 185 | 7.58 | 4% |
| Sample 3 | 416 | 441 | 428 | 481 | 441 | 24.45 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For CCL5 (Source: Uniprot.Org, NCBI)
Gene Name
CCL5
Full Name
C-C motif chemokine 5
Weight
9.99kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES CCL5 D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP C-C motif chemokine ligand 5 C-C motif chemokine 5|T-cell specific protein p288|beta-chemokine RANTES|chemokine (C-C motif) ligand 5|eosinophil chemotactic cytokine|regulated upon activation, normally T-expressed, and presumably secreted|small inducible cytokine subfamily A (Cys-Cys), member 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL5, check out the CCL5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Rantes ELISA Kit PicoKine® (EK0495)
Hello CJ!
EK0495 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Overexpression of mclca3 in airway epithelium of asthmatic murine models with airway inflammation
Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway
Electronic Cigarettes Induce Mitochondrial DNA Damage and Trigger TLR 9 (Toll-Like Receptor 9)-Mediated Atherosclerosis
Immunomodulatory effect of protease hydrolysates from ovotransferrin
Systemic delivery of TNF-armed myxoma virus plus immune checkpoint inhibitor eliminates lung metastatic mouse osteosarcoma
Indirectly stimulation of DCs by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model based on RNA-seq
Structural characteristics and immunopotentiation activity of two polysaccharides from the petal of Crocus sativus
Polysaccharide purified from Ganoderma atrum induced activation and maturation of murine myeloid-derived dendritic cells
Pristimerin Inhibits LPS-Triggered Neurotoxicity in BV-2 Microglia Cells Through Modulating IRAK1/TRAF6/TAK1-Mediated NF-κB and AP-1 Signaling Pathways In Vitro
Dexamethasone sodium phosphate attenuates lipopolysaccharide-induced neuroinflammation in microglia BV2 cells
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
11 Customer Q&As for Mouse Rantes ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-10-31
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-10-31
Question
Q: if the enzyme conjugated CCL5 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL5 antigen?
E. Scott
Verified customer
Asked: 2020-08-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-08-26
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-06-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-06-17
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-03-12
Question
Q: how to thaw whole blood sample for CCL5 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL5 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-01-29
Question
What is the dimension/measurement of the EK0495 plates?
Verified customer
Asked: 2019-10-29
Answer
The plates in the Mouse Rantes ELISA Kit PicoKine® (EK0495) measure 12.5cm in length, 8.5cm width, 1.4cm height. Diameter of well is 0.8cm.
Boster Scientific Support
Answered: 2019-10-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-09-25
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CCL5 in Mouse plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-05-19
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CCL5 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-05-19
Question
Q: how to analyze ELISA data? I measured CCL5 level in plasma.
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-12-26
Question
Q: What is the optimal O.D. value for CCL5 ELISA kit? I performed your CCL5 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CCL5 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL5 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of CCL5 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2018-05-07
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CCL5 using the EK0495 Mouse CCL5 Picokine® ELISA Kit?
A. Krishna
Verified customer
Asked: 2015-07-14
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2015-07-14
