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Product Info Summary
| SKU: | EK0504 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse sL-Selectin ELISA Kit PicoKine®
View all L-Selectin/CD62L ELISA kits
SKU/Catalog Number
EK0504
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse sL-Selectin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Sell in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse sL-Selectin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0504)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: W39-N332
Sensitivity
<5 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0504 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0504 is reactive to SELL in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0504 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of L-Selectin/CD62L
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0504-CAP | Anti-Mouse SELL Pre-coated 96-well strip microplate | 1 |
| EK0504-ST | Mouse SELL Standard | 2 vials, 10 ng/tube |
| EK0504-DA | Mouse SELL Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.002 | 0.037 | 0.074 | 0.129 | 0.311 | 0.527 | 1.177 | 1.804 |
Data Example Images
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Mouse L-Selectin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse sL-Selectin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 190 | 621 | 1771 | 204 | 669 | 1831 |
| Standard deviation | 9.5 | 32.29 | 138.13 | 11.01 | 34.78 | 157.46 |
| CV (%) | 5% | 5.2% | 7.8% | 5.4% | 5.2% | 8.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of L-Selectin/CD62L in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 190 | 188 | 188 | 191 | 189 | 1.29 | 0.6% |
| Sample 2 | 621 | 646 | 683 | 628 | 644 | 24.02 | 3.7% |
| Sample 3 | 1771 | 1866 | 1906 | 1738 | 1820 | 68.25 | 3.7% |
Protein Target Info & Infographic
Gene/Protein Information For SELL (Source: Uniprot.Org, NCBI)
Gene Name
SELL
Full Name
L-selectin
Weight
42.187kDa
Superfamily
selectin/LECAM family
Alternative Names
CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1 SELL CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1 selectin L L-selectin|CD62 antigen-like family member L|gp90-MEL|leukocyte surface antigen Leu-8|leukocyte-endothelial cell adhesion molecule 1|lymph node homing receptor|lymphocyte adhesion molecule 1|pln homing receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SELL, check out the SELL Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SELL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse sL-Selectin ELISA Kit PicoKine® (EK0504)
Hello CJ!
EK0504 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhong M, He B, Yang J, Bao R, Zhang Y, Zhou D, Chen Y, Li L, Han C, Yang Y, Sun Y, Cao Y, Li Y, Shi W, Jiang S, Zhang X, Yan H. Antimicrob Agents Chemother. 2012 Jun;56(6):3121-32. Doi: 10.1128/Aac.05950-11. Epub 2012 Mar 5. L-Selectin And P-Selec...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
7 Customer Q&As for Mouse sL-Selectin ELISA Kit PicoKine®
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
G. Young
Verified customer
Asked: 2020-03-20
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-03-20
Question
Q: What is the optimal O.D. value for CD62L ELISA kit? I performed your CD62L ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD62L even though the O.D. values are not very high?
Y. Evans
Verified customer
Asked: 2019-08-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD62L ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of CD62L that can be declared/interpreted as positive by the above standard is 5pg/ml.
Boster Scientific Support
Answered: 2019-08-07
Question
Q: how can I thaw whole blood sample for CD62L ELISA after freezing?
M. Tiwari
Verified customer
Asked: 2018-08-26
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD62L for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-08-26
Question
Q: Can CD62L ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-08-17
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-08-17
Question
Q: how to proceed with the analysis of ELISA data? I have performed CD62L level in serum.
B. Huang
Verified customer
Asked: 2018-06-13
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-06-13
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
G. Surve
Verified customer
Asked: 2018-06-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-06-05
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-05-23
