Click image to see more details
Product Info Summary
| SKU: | EK1754 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). |
Product info
Product Name
Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine®
View all TWEAK R/TNFRSF12 ELISA kits
SKU/Catalog Number
EK1754
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf12a in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1754)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: E28-P80
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1754 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1754 is reactive to Tnfrsf12a in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Application Guarantee
EK1754 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TWEAK R/TNFRSF12
The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. This gene is mapped to 17; 17 A3.3. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1754-CAP | Anti-Mouse Tnfrsf12a Pre-coated 96-well strip microplate | 1 |
| EK1754-ST | Mouse Tnfrsf12a Standard | 2 vials, 10 ng/tube |
| EK1754-DA | Mouse Tnfrsf12a Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.024 | 0.195 | 0.326 | 0.544 | 1.077 | 1.689 | 2.336 | 2.436 |
Data Example Images
Click image to see more details
Mouse TNFRSF12A/TWEAKR PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TWEAK R/TNFRSF12, Dilution ratio of 1:1, concentration in serum and plasma is around 2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 300 | 2167 | 4789 | 307 | 2379 | 4841 |
| Standard deviation | 18.3 | 143.02 | 258.6 | 13.2 | 114.19 | 363.08 |
| CV (%) | 6.1% | 6.6% | 5.4% | 4.3% | 4.8% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TWEAK R/TNFRSF12 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 300 | 325 | 308 | 330 | 315 | 12.21 | 3.8% |
| Sample 2 | 2167 | 2176 | 2072 | 2103 | 2129 | 43.52 | 2.0% |
| Sample 3 | 4789 | 4265 | 4165 | 4933 | 4538 | 328.89 | 7.2% |
Protein Target Info & Infographic
Gene/Protein Information For Tnfrsf12a (Source: Uniprot.Org, NCBI)
Gene Name
Tnfrsf12a
Full Name
Tumor necrosis factor receptor superfamily member 12A
Weight
13.641kDa
Alternative Names
CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14 Tnfrsf12a|AI255180, C87282, Fn1, Fn14, HPIP, TWEAK, TWEAK-R, Twea, TweakR|tumor necrosis factor receptor superfamily, member 12a|tumor necrosis factor receptor superfamily member 12A|FGF-inducible 14|fibroblast growth factor-inducible immediate-early response protein 14|fibroblast growth factor-regulated protein 2|tweak-receptor|type I transmembrane protein Fn14
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tnfrsf12a, check out the Tnfrsf12a Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnfrsf12a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine® (EK1754)
Hello CJ!
No publications found for EK1754
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
K. Roberts
Verified customer
Asked: 2020-03-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-28
Question
Q: if the enzyme conjugated TWEAK R antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TWEAK R antigen?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-03-16
Question
Q: is it okay to use EDTA plasma as samples in Mouse TWEAK R Picokine® ELISA Kit (Catalog # EK1754)?
H. Williams
Verified customer
Asked: 2019-11-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TWEAK R causing degradation of its protein structure. TWEAK R may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TWEAK R ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-11-15
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
A. Mitchell
Verified customer
Asked: 2019-03-03
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-03-03
Question
Q: how to proceed with the analysis of ELISA data? I have obtained TWEAK R level in serum.
T. Campbell
Verified customer
Asked: 2019-01-26
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-01-26
Question
Q: What is the optimal O.D. value for TWEAK R ELISA kit? I used your TWEAK R ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TWEAK R even though the O.D. values are not very high?
R. Kulkarni
Verified customer
Asked: 2018-05-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TWEAK R ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of TWEAK R that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2018-05-27
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-01-23
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-01-23
